Identification, expression and bioactivity of Paramisgurnus dabryanus beta-defensin that might be involved in immune defense against bacterial infection

文献类型： 外文期刊

第一作者： Chen, Yeyu

作者： Chen, Yeyu;Zhao, Heng;Zhang, Xinshang;Luo, Huiying;Xue, Xianli;Li, Zhongyuan;Yao, Bin

作者机构：

关键词： Paramisgurnus dabryanus;β-Defensin;Anti-bacterial activity;Inducible expression

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

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年卷期：

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收录情况： SCI

摘要： beta-defensins are a large family of multi-disulfide-bonded peptides with broad-spectrum antimicrobial activities that contribute to innate host defense in many organisms, but little information is available about beta-defensins produced by freshwater fish lacking scales. We therefore cloned and identified a beta-defensin gene from Chinese loach (Paramisgurnus dabryanus) by designing degenerate primers and using thermal asymmetric interlaced PCR. This gene is the first defensin gene ever identified in a non-scaled freshwater fish. Annotation of the protein domain architecture showed that the putative Chinese loach beta-defensin contains the signature motif of six conserved cysteines within the mature peptide, an aspect similar to beta-defensins of other marine fish. We also used quantitative real-time PCR to investigate the expression pattern of the Chinese loach beta-defensin gene, mRNA of which could be observed in various tissues. After challenge with the pathogenic bacterium Aeromonas hydrophila, beta-defensin expression was induced in the eye, gill, skin, and spleen of the adult loach. The bioactivity of the recombinant P. dabryanus beta-defensin was examined against pathogenic bacteria, and the results suggest that this class 2 beta-defensin has potential applications for treatment of bacterial infections

分类号： S9

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