Transgenic expression of an insect diapause-specific peptide (DSP) in Arabidopsis resists phytopathogenic fungal attacks

文献类型: 外文期刊

第一作者: Wu, Tingquan

作者: Wu, Tingquan;Lin, Yu'e;Liang, Zhaojun;Wu, Tingquan;Chen, Yongfang;Chen, Weida;Zou, Shenghao;Zhang, Yongqing;Tang, Dingzhong

作者机构:

关键词: Diapause-specific peptide;Antimicrobial peptide;Phytopathogenic fungus;Transgenic expression

期刊名称:EUROPEAN JOURNAL OF PLANT PATHOLOGY ( 影响因子:1.907; 五年影响因子:2.022 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Diapause-specific peptide (DSP) is an insect antimicrobial peptide, composed of 41 amino acid residues including six cysteines and isolated from diapausing adults of the leaf beetle Gastrophysa atrocyaneais. Recent research results have been demonstrated that in vitro, DSP has selective antifungal activity against the higher animal pathogenic fungus Trichophyton rubrum and the plant phytopathogenic fungus Fusarium solani. To elucidate the effect of DSP on other phytopathogenic fungi, we respectively introduced a Cht1SP-DSP-FLAG fusion gene and Cht1SP-FLAG fusion gene into Arabidopsis ecotype col-0. Transgenic plants expressing Cht1SP-DSP-FLAG fusion gene showed significant resistance to Golovinomyces cichoracearum (biotrophic fungus), Botrytis cinerea (necrotrophic fungus) and Hyaloperonospora arabidopsidis (H. a.) Noco2 (oomycete), whilst transgenic plants expressing Cht1SP-FLAG fusion gene showed no resistance to these pathogens. These results indicated that for many plant pathogenic fungi, DSP has high antimicrobial activity, which suggests that DSP has a huge potential in protecting crops from damage from phytopathogenic fungi.

分类号: S432.1

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