Reverse mutation of the virulence-associated S2 gene does not cause an attenuated equine infectious anemia virus strain to revert to pathogenicity

文献类型： 外文期刊

第一作者： Gao, Xu

作者： Gao, Xu;Jiang, Cheng-Gang;Wang, Xue-Feng;Lin, Yue-Zhi;Ma, Jian;Han, Xiu-E;Zhao, Li-Ping;Shen, Rong-Xian;Xiang, Wen-Hua;Zhou, Jian-Hua;Gao, Xu;Han, Xiu-E

作者机构：

关键词： EIAV;Pathogenicity;Reverse mutation;S2

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The contribution of S2 accessory gene of equine infectious anemia virus (EIAV) to the virulence of pathogenic strains was investigated in the present study by reverse mutation of all four consensus S2 mutation sites in an attenuated EIAV proviral strain, FDDV3-8, to the corresponding sequences of a highly pathogenic strain DV117. The S2 reverse-mutated recombinant strain FDDVS2r1-2-3-4 replicated with similar kinetics to FDDV3-8 in cultivated target cells. In contrast to the results of other studies of EIAV with dysfunctional S2, reverse mutation of S2 only transiently and moderately increased the plasma viral load of inoculated horses, and induction of transient immunosuppression did not boost viral pathogenicity. In addition, inoculation of FDDVS2r1-2-3-4 induced partial protection to a challenge pathogenic virus. These results suggest that the attenuated EIAV vaccine strain with multiple mutations in multiple genes will not easily revert to a virulent phenotype.

分类号： R37

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