High-yield production of a low-temperature-active polygalacturonase for papaya juice clarification

文献类型： 外文期刊

第一作者： Tu, Tao

作者： Tu, Tao;Meng, Kun;Bai, Yingguo;Shi, Pengjun;Luo, Huiying;Wang, Yaru;Yang, Peilong;Yao, Bin;Zhang, Yuhong;Zhang, Wei

作者机构：

关键词： Achaetomium sp. Xz8;Endo-polygalacturonase;Low-temperature-active;High specific activity;Juice clarification

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

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收录情况： SCI

摘要： A novel endo-polygalacturonase (endo-PG I) from Achaetomium sp. Xz8 was identified, overexpressed in Pichia pastoris, and characterized in this report. Recombinant endo-PG I is distinguished from other enzyme counterparts by its high activity towards polygalacturonic acid (49,934 U/ml) and high yield in the 15-l fermentor (2.13 g/l). It exhibits optimal activity at 45 ℃ and remained active over a broad temperature range of 0-80 ℃. Distinct from most fungal polygalacturonases that have acidic pH optima, endo-PG I is optimally active at pH 6, similar to the pH of fresh papaya juice (5.7). Endo-PG I alone reduced the viscosity of papaya juice by 17.6%, and increased its transmittance by 59.1%. When combined with a commercial pectin methylesterase, it showed much higher efficiency with a synergy degree of more than 1.25. All these favourable enzymatic properties make endo-PG I attractive for potential applications in the juice industry.

分类号： TS2`TS201.2

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