Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench)

文献类型： 外文期刊

第一作者： Gao, Jianming

作者： Gao, Jianming;Yuan, Qinghua;Li, Xianglin;Gao, Jianming;Xia, Buxian;Luo, Feng;Sun, Shoujun;Pei, Zhongyou;Gui, Zhi

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期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

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收录情况： SCI

摘要： A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS method and develop maintainer lines with sweet and juicy stalks and corresponding cytoplasmic-nuclear male sterility (CMS) lines. The simple sequence repeat marker Xtxp18 exhibited a high accuracy (95.098 %) for selecting recessive homozygotes for the rf1 gene. The segregation ratio matched the expected ratio calculated according to the reported genetic distance in the F-2 population of the two crosses used. Finally, four excellent maintainer lines/CMS line pairs (F-5/BC3) with high stalk juice and stalk juice sugar contents were developed. The MAS method based on Xtxp18 for the sorghum rf1 gene could be used for hybrid breeding programs at a low cost in the future.

分类号： S3

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