Identification and Binding Characterization of Three Odorant Binding Proteins and One Chemosensory Protein from Apolygus lucorum (Meyer-Dur)

文献类型： 外文期刊

第一作者： Jin-Feng Hua;;Shuai Zhang

作者： Jin-Feng Hua;Shuai Zhang;Jin-Jie Cui;Dao-Jie Wang;Chun-Yi Wang;Jun-Yu Luo;Li-Min Lv

作者机构：

期刊名称：JOURNAL OF CHEMICAL ECOLOGY （ 影响因子：2.626； 五年影响因子：2.984 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： For the sucking insect, Apolygus lucorum, taste is essential for finding host plants and oviposition sites. In A. lucorum, taste relies largely on the sensory system located within its proboscis. In this study, we constructed a cDNA library from A. lucorum proboscises and conducted preliminary analysis of 1554 ESTs. From this collection, we identified three putative odorant-binding proteins (AlucOBP3, AlucOBP4, AlucOBP6) and one chemosensory protein (AlucCSP1). Quantitative real-time polymerase chain reaction (qPCR) was used to study the expression pattern of these four genes. All four were expressed mainly in antennae, proboscises and legs, suggestive of roles in olfaction and gustation. We expressed and purified recombinant versions of AlucOBP3, AlucOBP4, AlucOBP6, and AlucCSP1 in a prokaryotic expression system. The ligand-binding specificities of the four proteins then were investigated in competition assays using 4,4'-dianilino-1,1'-binaphthyl-5, 5'-sulfonic acid (bis-ANS) as a probe. Of the 58 small organic compounds and five cotton secondary metabolites tested, plant volatiles cannot effectively displace bis-ANS from any of the four proteins. In contrast, most of the cotton secondary metabolites have high affinities for the three OBPs and AlucCSP1, indicating that these binding proteins more likely play a role in gustation than in olfaction.

分类号： 58.18

  • 相关文献
作者其他论文 更多>>

微信小程序