Colorimetric and fluorometric dual-readout sensor for lysozyme

文献类型： 外文期刊

第一作者： Zheng, Hanye

作者： Zheng, Hanye;Qiu, Suyan;Xu, Kefeng;Lin, Zhenyu;Guo, Longhua;Qiu, Bin;Chen, Guonan;Qiu, Suyan;Luo, Linguang;Song, Yibiao

作者机构：

关键词： Colorimetric;lysozyme;detection

期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel, highly sensitive and selective dual-readout sensor (colorimetric and fluorometric) for the detection of lysozyme was proposed. The fluorescence of triazolylcoumarin molecules was quenched by Au nanoparticles (AuNPs) initially through the fluorescence resonance energy transfer (FRET), after the addition of lysozyme, the stronger binding of lysozyme onto the surfaces of AuNPs made triazolylcoumarin molecules remove from the AuNPs surface and led to the recovery of the fluorescence of triazolylcoumarin molecules, and accompanied by the discernable color change of the solution from red to purple. The lowest detectable concentration for lysozyme was 50 ng mL~(-1) by the naked eye, and the limit of detection (LOD) was 23 ng mL~(-1) by fluorescence measurements. In addition, satisfactory results for lysozyme detection in hen egg white were confirmed in the study. Moreover, the presented sensor provides a reliable option to determine lysozyme with high sensitivity and selectivity.

分类号： O65

  • 相关文献

[1]Colorimetric Sensing of Cysteine in serum by the competitive adsorption toward AuNPs between Cysteine and ATP. Zhao, Lingzhi,Miao, Yanqing,Liu, Chunye,Zhao, Liu,Zhao, Lingzhi. 2015

[2]Colorimetric Detection of Cr3+ in Aqueous Solution Based on Cofunctionalized Silver Nanoparticles Modified with 4-Nitrobenzenethiol and 4-Mercaptobenzoic Acid. Zhang, Zhikun,Zhou, Ying,Yang, Jing-Kui,Zhao, Hong,He, Yujian,Wang, Peilong,He, Yujian,Cao, Zhiqin,Luo, Maoqiang.

[3]Colorimetric detection of ractopamine and salbutamol using gold nanoparticles functionalized with melamine as a probe. Wang, Peilong,Su, Xiaoou,Zhou, Ying,Zhao, Hong,He, Yujian,He, Yujian.

[4]Use of a novel metal indicator to judge loop-mediated isothermal amplification for detecting the 35S promoter. Wang, Xiaofu,Chen, Xiaoyun,Peng, Cheng,Xu, Xiaoli,Wei, Wei,Xu, Junfeng,Wang, Xiaofu,Chen, Xiaoyun,Peng, Cheng,Xu, Xiaoli,Wei, Wei,Xu, Junfeng,Fu, Zhenfang,Li, Feiwu.

[5]Purification and properties of lysozyme from a marine strain. Zhang, Xiu,Ren, Xian,Zhang, Xiu,Wang, Yue-Jun,Sun, Mi,Zou, Yan-Li,Wang, Qing-Yin,Wang, Wei. 2008

[6]Dilational rheology of different globular protein with imidazolium-based ionic liquid surfactant adsorption layer at the decane/water interface. Zhou, Zhao-Lu,Zheng, Li,Huang, Qi-Liang,Du, Feng-Pei. 2017

[7]Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila. Ardo, Laszlo,Varadi, Laszlo,Jeney, Zsigmond,Jeney, Galina,Yin, Guojun,Xu, Pao,Szigeti, Gabor.

[8]Preparation and evaluation of lysozyme-loaded nanoparticles coated with poly-gamma-glutamic acid and chitosan. Liu, Yong,Xu, Yaoxing,Tang, Jiangwu,Liu, Wei,Sun, Dongchang,Jiang, Hua,Xu, Shaochun,Sun, Yan,Fu, Sida,Feng, Hai. 2013

[9]Effect of high dietary carbohydrate on growth, serum physiological response, and hepatic heat shock cognate protein 70 expression of the top-mouth culter Erythroculter ilishaeformis Bleeker. Liu, Bo,Xie, Jun,Ge, Xian-ping,Wang, Guangyu,Liu, Bo,Xie, Jun,Ge, Xian-ping,Miao, Ling-hong.

[10]Nanogels fabricated by lysozyme and sodium carboxymethyl cellulose for 5-fluorouracil controlled release. Zhu, Kunkun,Ye, Ting,Liu, Jinjin,Xu, Shasha,Lei, Jieqiong,Deng, Hongbing,Li, Bin,Zhu, Kunkun,Ye, Ting,Liu, Jinjin,Xu, Shasha,Lei, Jieqiong,Deng, Hongbing,Li, Bin,Zhu, Kunkun,Peng, Zheng.

[11]Immune responses and expression of immune-related genes in swimming crab Portunus trituberculatus exposed to elevated ambient ammonia-N stress. Yue, Feng,Pan, Luqing,Xie, Peng,Zheng, Debin,Li, Jian.

[12]Expression of Apostichopus japonicus lysozyme in the methylotrophic yeast Pichia pastoris. Wang, Tingting,Xu, Yongping,Liu, Wenjia,Jin, Liji,Xu, Yongping,Jin, Liji,Sun, Yongxin,Wang, Tingting,Xu, Yongping,Liu, Wenjia,Sun, Yongxin,Jin, Liji.

[13]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[14]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[15]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[16]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[17]Rapid detection of porcine parvovirus DNA by sensitive loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Yang, Sheng-hai,Ma, Li-na,Ma, Yan-ping,Liu, Xiang-tao,Cai, Xue-peng,Zhang, Yong-guang,Liu, Yong-sheng. 2009

[18]Rapid detection of porcine circovirus type 2 by loop-mediated isothermal amplification. Chen, Hao-tai,Zhang, Jie,Sun, De-hui,Chu, Yue-feng,Cai, Xue-peng,Liu, Xiang-tao,Luo, Xue-nong,Liu, Yong-sheng,Liu, Qing. 2008

[19]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[20]Quantitative detection of the rice false smut pathogen Ustilaginoidea virens by real-time PCR. Li, H.,Ni, D. H.,Li, J.,Li, H.,Ni, D. H.,Duan, Y. B.,Li, J.,Song, F. S.,Li, L.,Wei, P. C.,Yang, J. B.,Li, H.,Wei, P. C.,Duan, Y. B.,Song, F. S.,Chen, Y.. 2013

作者其他论文 更多>>

微信小程序