Sensitive competitive immunoassay of multiple mycotoxins with non-fouling antigen microarray
文献类型: 外文期刊
第一作者: Hu, Weihua
作者: Hu, Weihua;He, Guangli;Zheng, Xinting;Li, Chang Ming;Hu, Weihua;He, Guangli;Zheng, Xinting;Li, Chang Ming;Li, Xin;Zhang, Zhaowei;Li, Peiwu
作者机构:
关键词: Antigen microarray;Competitive immunoassay;Mycotoxins;Polymer brush
期刊名称:BIOSENSORS & BIOELECTRONICS ( 影响因子:10.618; 五年影响因子:9.323 )
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收录情况: SCI
摘要: Various mycotoxins with strong carcinogenesis and toxicity are fatal threats in food safety, and require highly sensitive and high-throughput detections greatly. Herein a fluorescent competitive immunoassay microarray based on a non-fouling polymer brush, poly[(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) is explored to sensitively detect multiple mycotoxins with aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEN) as template targets. Due to uniformly large protein loading and high resistance to nonspecific protein absorption of the POEGMA-co-GMA brush, the optimal microarray exhibits wide dynamic ranges of three orders of magnitudes and low detection limits of 4, 4 and 3pgmL~(-1), respectively, which is much better than that obtained with an epoxy-functionalized antigen microarray, and is comparable or even better than the conventional ELISA method. This work offers a powerful high-throughput tool to fast screening of toxins in food quality and environmental monitoring.
分类号: Q
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