A universal DNA-based protein detection system

文献类型： 外文期刊

第一作者： Tran, Thua N. N.

作者： Tran, Thua N. N.;Cui, Jinhui;Hartman, Mark R.;Peng, Songming;Funabashi, Hisakage;Duan, Faping;Yang, Dayong;March, John C.;Luo, Dan;Lis, John T.;Luo, Dan;Cui, Jinhui;Cui, Haixin;Funabashi, Hisakage

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关键词： detection requires;DNA-based;high capacity

期刊名称：JOURNAL OF THE AMERICAN CHEMICAL SOCIETY （ 影响因子：15.419； 五年影响因子：15.801 ）

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收录情况： SCI

摘要： Protein immune detection requires secondary antibodies which must be carefully selected in order to avoid interspecies cross-reactivity, and is therefore restricted by the limited availability of primary/secondary antibody pairs. Here we present a versatile DNA-based protein detection system using a universal adapter to interface between IgG antibodies and DNA-modified reporter molecules. As a demonstration of this capability, we successfully used DNA nano-barcodes, quantum dots, and horseradish peroxidase enzyme to detect multiple proteins using our DNA-based labeling system. Our system not only eliminates secondary antibodies but also serves as a novel method platform for protein detection with modularity, high capacity, and multiplexed capability.

分类号： O6

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