Detection of Acute Toxoplasmosis in Pigs Using Loop-Mediated Isothermal Amplification and Quantitative PCR

文献类型： 外文期刊

第一作者： Wang, Yanhua

作者： Wang, Yanhua;Wang, Guangxiang;Wang, Meng;Zhang, Delin;Yin, Hong

作者机构：

关键词： Toxoplasma gondii;loop-mediated isothermal amplification (LAMP);Q-PCR;pig

期刊名称：KOREAN JOURNAL OF PARASITOLOGY （ 影响因子：1.341； 五年影响因子：1.669 ）

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收录情况： SCI

摘要： A loop-mediated isothermal amplification (LAMP) assay allows rapid diagnosis of Toxoplasma gondii infection. In the present study, the LAMP assay was evaluated using blood from both naturally and experimentally infected pigs. The sensitivity of the LAMP assay was compared with that of Q-PCR. Both assays detected T gondii in the blood of experimentally infected pigs, with 100% agreement. In infected blood samples, the parasite was detected as early as 2 days post-infection and reached a peak in 3-5 days. In 216 field serum samples, the detection rates of LAMP and Q-PCR assays were 6.9% and 7.8%, respectively. This result indicates that the sensitivity of the LAMP assay was slightly lower than that of the Q-PCR assay. However, the LAMP may be an attractive diagnostic method in conditions where sophisticated and expensive equipment is unavailable. This assay could be a powerful supplement to current diagnostic methods.

分类号： Q18

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