Selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time PCR

文献类型： 外文期刊

第一作者： Zhang, Kun

作者： Zhang, Kun;Niu, Shaofang;Shi, Lindan;Liu, Deshui;Cao, Xiuling;Wang, Xianbing;Han, Chenggui;Yu, Jialin;Li, Dawei;Zhang, Yongliang;Zhang, Kun;Niu, Shaofang;Shi, Lindan;Liu, Deshui;Cao, Xiuling;Wang, Xianbing;Han, Chenggui;Yu, Jialin;Li, Dawei;Zhang, Yongliang;Di, Dianping;Miao, Hongqin

作者机构：

关键词： qPCR;Reference gene;Monocots;Viral infection;PR-1

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Both genome-wide transcriptomic surveys of the mRNA expression profiles and virus-induced gene silencing-based molecular studies of target gene during virus-plant interaction involve the precise estimation of the transcript abundance. Quantitative real-time PCR (qPCR) is the most widely adopted technique for mRNA quantification. In order to obtain reliable quantification of transcripts, identification of the best reference genes forms the basis of the preliminary work. Nevertheless, the stability of internal controls in virus-infected monocots needs to be fully explored. In this work, the suitability of ten housekeeping genes (ACT, EF1 alpha, FBOX, GAPDH, GTPB, PP2A, SAND, TUB beta UBC18 and UK) for potential use as reference genes in qPCR were investigated in five different monocot plants (Brachypodium, barley, sorghum, wheat and maize) under infection with different viruses including Barley stripe mosaic virus (BSMV), Brome mosaic virus (BMV), Rice black-streaked dwarf virus ( RB S DV) and Sugarcane mosaic virus (SCMV). By using three different algorithms, the most appropriate reference genes or their combinations were identified for different experimental sets and their effectiveness for the normalisation of expression studies were further validated by quantitative analysis of a well-studied PR-1 gene. These results facilitate the selection of desirable reference genes for more accurate gene expression studies in virus-infected monocots

分类号： Q81

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