The effectiveness of ISSR profiling for studying genetic diversity of Aspergillus flavus from peanut-cropped soils in China

文献类型： 外文期刊

第一作者： Zhang, Chu-Shu

作者： Zhang, Chu-Shu;Xing, Fu-Guo;Selvaraj, Jonathan Nimal;Zhou, Lu;Zhao, Yue-Ju;Liu, Yang;Zhang, Chu-Shu;Yang, Qing-Li

作者机构：

关键词： Inter-simple sequence repeats (ISSR);Aspergillus flavus;Primer screening;Genetic diversity

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

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收录情况： SCI

摘要： We used markers based on inter-simple sequence repeats (ISSR) to examine the genetic diversity of Aspergillus flavus from peanut-cropped soils in China. Of the 100 primers, 22 primers produced clear and reproducible ISSR bands, and the di-nucleotide accounted for 73% of those primers. The size of DNA fragments ranged from 100 to 2000 bp. The primer UBC 834 produced the largest number of polymorphic bands (10), followed by UBC 809, UBC 817, UBC 895, and UBC 899, which all amplified 7 polymorphic bands. Using the five primers, the tested strains were clearly separated based on genetic similarity coefficients (GSC). The range of GSC was from 0.59 to 0.90. In unweighted pair-group method with arithmetic averages (UPGMA) analysis, the A. flavus samples grouped in five clusters. The study showed that the ISSR technology is an effective molecular approach for studying diversity of A. flavus from peanut-cropped soils in China

分类号： Q5

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