Molecular characterization and functional analysis of a specific secreted protein from highly virulent defoliating Verticillium dahliae

文献类型： 外文期刊

第一作者： Liu, Shao-Yan

作者： Liu, Shao-Yan;Chen, Jie-Yin;Wang, Jin-Long;Li, Lei;Xiao, Hong-Li;Adam, Sami M.;Dai, Xiao-Feng

作者机构：

关键词： Pathogenicity;Plant cell wall degradation;Secreted protein;V. dahliae;VdSSP1

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Verticillium dahliae Kleb. is a phytopathogenic fungus that causes wilt diseases in hundreds of dicotyledonous plant species. Previous research has demonstrated that the secretome plays an important role in the pathogenicity of V. dahliae. In this study, the specific secreted protein gene (VdSSP1) in highly virulent defoliating V. dahliae strain VDG1 was cloned, and considered to be a secreted protein by signal peptide activity assay. VdSSP1 deletion mutants in VDG1 significantly compromised virulence, and the fungal growth decreased in media with pectin and starch as carbon sources. Pathogenicity and carbon utilization were restored upon complementation of the VdSSP1 deletion strains or low virulence non-defoliating strain VDG2, which lacks VdSSP1. It is indicated that the virulence role of VdSSP1 is associated with plant cell wall degradation. In conclusion, our data suggested that VdSSP1 is a secreted protein that is engaged in the pathogenicity of the highly virulent defoliating V. dahliae.

分类号： R394

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