Isolation of an indigenous imidacloprid-degrading bacterium and imidacloprid bioremediation under simulated In Situ and Ex Situ conditions

文献类型： 外文期刊

第一作者： Hu, Guiping

作者： Hu, Guiping;Zhao, Yan;Song, Fengqing;You, Minsheng;Liu, Bo

作者机构：

关键词： Bioremediation;Imidacloprid;Microorganism community;Ochrobactrum sp.

期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：2.351； 五年影响因子：2.65 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. The dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, Rhizobium sp., Sinorhizobium, Ochrobactrum sp., Alcaligenes, Bacillus sp., Bacterium, Klebsiella sp., and Ensifer adhaerens. The bacterial community structure was altered extensively and its complexity reduced during the enrichment process, and four culturable bacteria, Ochrobactrum sp., Rhizobium sp., Geobacillus stearothermophilus, and Alcaligenes faecalis, remained in the final enrichment. Only one indigenous strain, BCL-1, with imidacloprid-degrading potential, was isolated from the sixth enrichment culture. This isolate was a gram-negative rod-shaped bacterium and identified as the genus Ochrobactrum based on its morphological, physiological, and biochemical properties and its 16S rRNA gene sequence. The degradation test showed that approximately 67.67% of the imidacloprid (50 mg/l) was degraded within 48 h by strain BCL-1. The optimum conditions for degradation were a pH of 8 and 30°C. The simulation of imidacloprid bioremediation by strain BCL-1 in soil demonstrated that the best performance in situ (tea soil) resulted in the degradation of 92.44% of the imidacloprid (100 mg/g) within 20 days, which was better than those observed in the ex situ simulations that were 64.66% (cabbage soil), 41.15% (potato soil), and 54.15% (tomato soil).

分类号： Q93

  • 相关文献

[1]Protective function of narrow grass hedges on soil and water loss on sloping croplands in Northern China. Xiao, Bo,Wang, Qing-hai,Wu, Ju-ying,Huang, Chuan-wei,Yu, Ding-fang,Xiao, Bo.

[2]INFLUENCE OF THE Nocardia sp. C-14-1 REMEDIATION ON THE GROWTH OF POTTED PLANTS IN CONTAMINATED SOIL AND ON MICROORGANISM BIO-DIVERSITY. Ma, Hai-Juan,Ma, Hai-Juan,Li, Guang-Ming,Zhang, Ya-Lei,Fang, Ping. 2011

[3]Bioremediation of Eutrophic Water by the Controlled Cultivation of Water Hyacinths. Zhang, Yingying,Zhang, Zhiyong,Wang, Zhi,Liu, Haiqin,Wen, Xuezheng,Qin, Hongjie,Yan, Shaohua,Zhang, Yingying,Rittmann, Bruce E.. 2016

[4]Bioremediation of the tobacco waste-contaminated soil by Pseudomonas sp HF-1: nicotine degradation and microbial community analysis. Wang, Xin,Tang, Lu,Wang, Haixia,Min, Hang,Lu, Zhenmei,Yao, Yanlai. 2013

[5]Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19. Wang, Y.,Gao, J.,Wang, C.,Li, A..

[6]Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.. Xu, Gangming,Li, Yingying,Zheng, Wei,Peng, Xiang,Li, Wen,Yan, Yanchun.

[7]Characterization of cadmium-resistant bacteria and their potential for reducing accumulation of cadmium in rice grains. Chen, Mingxue.

[8]Phytodegradation of organophosphorus compounds by transgenic plants expressing a bacterial organophosphorus hydrolase. Wang, Xiaoxue,Wu, Ningfeng,Guo, Jun,Chu, Xiaoyu,Tian, Jian,Fan, Yunliu,Yao, Bin.

[9]Ecological engineering in aquaculture - Potential for integrated multi-trophic aquaculture (IMTA) in marine offshore systems. Troell, Max,Joyce, Alyssa,Troell, Max,Joyce, Alyssa,Joyce, Alyssa,Chopin, Thierry,Chopin, Thierry,Neori, Amir,Buschmann, Alejandro H.,Fang, Jian-Guang. 2009

[10]Fast atrazine degradation by the mixed cultures enriched from activated sludge and analysis of their microbial community succession. Zhou, Qingxin,Chen, Leilei,Wang, Junhua,Qiu, Jiying,Liu, Xiaoyong,Zhang, Xiang,Chen, Xiangyan,Wang, Zhibin,Ni, Shouqing,Wang, Zhibin.

[11]Genome Sequence of Acinetobacter calcoaceticus PHEA-2, Isolated from Industry Wastewater. Zhan, Yuhua,Yan, Yongliang,Zhang, Wei,Yu, Haiying,Chen, Ming,Lu, Wei,Ping, Shuzhen,Peng, Zixin,Yuan, Menglong,Zhou, Zhengfu,Lin, Min,Elmerich, Claudine.

[12]Expression, purification and functional characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from Rhodococcus rhodochrous. Xiong, Fei,Shuai, Jian-Jun,Peng, Ri-He,Tian, Yong-Sheng,Zhao, Wei,Yao, Quan-Hong,Xiong, Ai-Sheng,Xiong, Fei,Shuai, Jian-Jun.

[13]Bioremediation potential of glyphosate-degrading Pseudomonas spp. strains isolated from contaminated soil. Zhu, Jianyi,Liu, Shengnan,Gao, Han,Zhou, Xiaogang,Tao, Ke.

[14]Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp YC-RL4 and the bioprocess with environmental samples. Ren, Lei,Jia, Yang,Ruth, Nahurira,Qiao, Cheng,Wang, Junhuan,Zhao, Baisuo,Yan, Yanchun.

[15]Application of Gracilaria lichenoides (Rhodophyta) for alleviating excess nutrients in aquaculture. Xu, Yongjian,Wei, Wei,Fang, Jianguang.

[16]Starch-enhanced degradation of HMW PAHs by Fusarium sp in an aged polluted soil from a coal mining area. Zhao, Ou-ya,Zhang, Xue-na,Feng, Sheng-Dong,Zhang, Li-Xiu,Shi, Wei,Yang, Zhi-xin,Chena, Miao-Miao,Fanga, Xue-Dan,Zhao, Ou-ya,Zhang, Xue-na,Feng, Sheng-Dong,Zhang, Li-Xiu,Shi, Wei,Yang, Zhi-xin,Chena, Miao-Miao,Fanga, Xue-Dan,Zhao, Ou-ya.

[17]Fates of nickel and fluoranthene during the bioremediation by Pleurotus eryngii in three different soils. Shi, Wenjin,Gao, Ni,Zuo, Lei,Xu, Heng,Dong, Shunwen.

[18]Arsenic removal from contaminated soil via biovolatilization by genetically engineered bacteria under laboratory conditions. Liu, Shuang,Chen, Jian,Sun, Guoxin,Zhang, Fan.

[19]Influence of environmental factors on degradation of carbendazim by Bacillus pumilus strain NY97-1. Zhang, Lizhen,Qiao, Xiongwu,Ma, Liping,Zhang, Lizhen.

[20]Isolation, Colonization, and Chlorpyrifos Degradation Mediation of the Endophytic Bacterium Sphingomonas Strain HJY in Chinese Chives (Allium tuberosum). Feng, Fayun,Ge, Jing,Cheng, Jinjin,Yu, Xiangyang,Feng, Fayun,Ge, Jing,Zhong, Jianfeng,Yu, Xiangyang,Li, Yisong.

作者其他论文 更多>>

微信小程序