VP1 B-C and D-E loops of bovine enterovirus cluster B can effectively display foot-and-mouth disease virus type O-conserved neutralizing epitope

文献类型： 外文期刊

第一作者： Chang, Jitao

作者： Chang, Jitao;Li, Yingli;Yang, Decheng;Wang, Fang;Jiang, Zhigang;Yu, Li

作者机构：

关键词： VP1 B-C;O-conserved neutralizing;epitope

期刊名称：JOURNAL OF GENERAL VIROLOGY （ 影响因子：3.891； 五年影响因子：3.719 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： On the basis of generation of an infectious cDNA clone for the BHM26 strain of bovine enterovirus cluster B (BEV-B), 22 sites on different loops of the BHM26 capsid were selected according to an alignment of its sequence with the structural motifs of BEV-A strain VG-5-27 for insertion of the foot-and-mouth disease virus (FMDV) type O-conserved neutralizing epitope 8E8. Two recombinant viruses, rBEV-A1 and rBEV-DE, in which the FMDV epitope was inserted into the VP1 B-C or D-E loops, were rescued by transfection of BHK-21 cells with the in vitrotranscribed RNA of the recombinant BHM26 genome-length cDNA constructs. The two epitopeinserted viruses were genetically stable and exhibited growth properties similar to those of their parental virus in BHK-21 and IBRS-2 cells, which are susceptible to both BEV and FMDV. However, the two recombinant BEVs (rBEVs) had a significantly lower growth titre than those of the parental virus BHM26 in MDBK and Marc145 cells, which are susceptible to BEV but not to FMDV. These results indicated that insertion of the FMDV epitope into the VP1 B-C or D-E loops of the BEV particle altered the replication properties of BEV. In addition, the two rBEVs were sensitive to neutralization by the FMDV type O-specific mAb 8E8, and anti-FMDV IgG antibodies were induced in mice by intramuscular inoculation with the rBEV-A1 and rBEV-DE viruses. Our results demonstrate that the VP1 B-C and D-E loops of the BEV-B particle can effectively display a foreign epitope, making this an attractive approach for the design of BEV-vectored and epitope-based vaccines.

分类号： R37

  • 相关文献

[1]Antigenic structure analysis of VP3 of infectious bursal disease virus. Deng, Xiaoyun,Gao, Yulong,Gao, Honglei,Qi, Xiaole,Cheng, Yu,Wang, Xiaoyan,Wang, Xiaomei. 2007

[2]Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon alpha in mice and protective efficacy in guinea pigs and swine. Du, Yijun,Li, Yufeng,Jiang, Wenming,Wang, Xinglong,Tang, Bo,Cao, Jun,Wang, Xianwei,Jian, Ping,He, Hairong,Qi, Jing. 2008

[3]Antigenic structure analysis of glycosylated protein 3 of porcine reproductive and respiratory syndrome virus. Zhou, YJ,An, TQ,He, YX,Liu, JX,Qiu, HJ,Wang, YF,Tong, G. 2006

[4]Anti-human fibroblast growth factor-21 monoclonal antibody preparation, characterization and analysis of in vitro bioactivity. Ding, Liangjun,Yuan, Qingyan,Xu, Pengfei,Yu, Yinhang,Li, Deshan,Hao, Zhichao. 2016

[5]Identification of two antigenic epitopes on SARS-CoV spike protein. Hua, RH,Zhou, YJ,Wang, YF,Hua, YZ,Tong, GZ. 2004

[6]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[7]SPIKE PROTEIN REGION (aa 636-789) OF PORCINE EPIDEMIC DIARRHEA VIRUS IS ESSENTIAL FOR INDUCTION OF NEUTRALIZING ANTIBODIES. Sun, D. B.,Feng, L.,Shi, H. Y.,Chen, J. F.,Liu, S. W.,Chen, H. Y.,Wang, Y. F..

[8]Monoclonal antibodies and conserved antigenic epitopes in the C terminus of GP5 protein of the North American type porcine reproductive and respiratory syndrome virus. Zhou, Yan-Jun,Yu, Hai,Li, Guo-Xin,Jiang, Yi-Feng,Tong, Guang-Zhi,Tian, Zhi-Jun,Liu, Jin-Xia,An, Tong-Qing,Peng, Jin-Mei,Cai, Xue-Hui,Wang, Mei,Wang, Yun-Feng,Xue, Qiang.

[9]Identifying Novel B Cell Epitopes within Toxoplasma gondii GRA6. Wang, Yanhua,Wang, Guangxiang,Cai, Jian Ping. 2016

[10]Phage display identifies an Eastern equine encephalitis virus glycoprotein E2-specific B cell epitope. Zhao, J.,Sun, E. C.,Liu, N. H.,Yang, T.,Xu, Q. Y.,Qin, Y. L.,Wu, D. L.,Zhao, J.,Sun, E. C.,Qin, Y. L.,Yang, Y. H.. 2012

[11]Screening and identification of novel B cell epitopes of Toxoplasma gondii SAG1. Wang, Yanhua,Wang, Guangxiang,Zhang, Delin,Yin, Hong,Zhang, Delin,Wang, Meng,Wang, Yanhua,Wang, Guangxiang,Zhang, Delin,Yin, Hong,Wang, Meng. 2013

[12]Critical Amino Acids Analysis of Epitopes in Pen a1. Mou Hui,Gao Mei-Xu,Wang Zhi-Dong,Zhao Jie,Li Shu-Jin,Zhao Xin,Li Shu-Rong,Pan Jia-Rong,Zhi Yu-Xiang. 2014

[13]Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus. Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Lei, Zhi-Hai,Zeng, Jiang-Yong,Zhu, Se,Duoji, Ciren,Dunzhu, Luobu. 2013

[14]Genotype-specific acquisition, evolution and adaptation of characteristic mutations in hepatitis E virus. Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua,Wang, Wenshi,Peppelenbosch, Maikel P.,Pan, Qiuwei,Ikram, Aqsa,Hakim, Mohamad S.,Zhou, Jian-hua. 2018

[15]Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174. Yu, Shenye,Zhao, Hailing,Wang, Huanan,Wang, Xiumei,Xu, Lili,Si, Wei,Chen, Liping,Zhang, Wanjiang,Liu, Siguo,Shao, Guangxi. 2013

[16]In silico analysis and recombinant expression of BamA protein as a universal vaccine against Escherichia coli in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua.

[17]Identification of a conserved B-cell epitope in the equine arteritis virus (EAV) N protein using the pepscan technique. Zhao, Shihua,Qi, Ting,Guo, Wei,Lu, Gang,Xiang, Wenhua.

[18]Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Ren, Jian-Le,Zhu, Yuan-Mao,Zhou, Yue-Hui,Lv, Chuang,Yan, Hao,Ma, Lei,Shi, Hong-Fei,Xue, Fei.

[19]Expression and antigenic epitopes mapping of receptor binding domain on the spike protein of severe acute respiratory syndrome coronavirus. Rua, RH,Tong, GZ,Wang, YF,Zhou, YJ. 2005

[20]Identification of a linear epitope on the capsid protein of classical swine fever virus. Zhang, Xin,Sun, Yuan,Li, Su,Li, Na,Yang, Shuo,He, Fan,Huang, Jun-Hua,Ling, Li-Jun,Qiu, Hua-Ji,Xu, Jia. 2011

作者其他论文 更多>>

微信小程序