Identification for Different Expression Proteins in Different Virulent Streptococcus suis Serotype 7 Strains

文献类型： 外文期刊

第一作者： Wang, Shujie

作者： Wang, Shujie;Li, Liqin;Liu, Siguo;Liu, Yonggang;Qi, Yingfang;Wang, Gang;Cai, Xuehui;Jiang, Yongqiang

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期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

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收录情况： SCI

摘要： Streptococcus suis (S. suis) secreted numerous proteins are virulence-associated factors that play critical roles m infection Studies of S. suis secreted proteins have been reported in S. suis Serotype 2 (SS2) strains and S. suis Serotype 9 (SS9) strains However, data is still scarce In this study, a comparative proteomics approach was used to distinguish the profiles of the secreted proteins m different virulent SS7 strains Consequently, 13 individual proteins that were unique to either high pathogenic 07WC11 strain or low pathogenic SJZB408 strain m 2D gels were successfully identified In addition, researchers attempted to compare the amount of expression 10 identified protein genes (pft, rsp, cms, esp, hpk, pk, rpp, rrf tef, tis) by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Real-time RT-PCR results showed that pft, rsp, cms, esp, pk, rpp, rrf and tef mRNA expression amount m low pathogenic SS7 strain was lower than in high pathogenic SS7 strain which is accord with the results of 2D Thus, researchers defined these eight proteins as different expression proteins between low pathogenic and high pathogenic SS7 strain

分类号： S

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