Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus)

文献类型： 外文期刊

第一作者： Wang, T. T.

作者： Wang, T. T.;Wang, N.;Liao, X. L.;Meng, L.;Liu, Y.;Chen, S. L.;Wang, T. T.

作者机构：

关键词： Turbot (Scophthalmus maximus);Hsc70;Heat shock;Bacterial infection;Embryogenesis

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： As an essential member of the HSP70 family, heat shock cognate 70 (Hsc70) is a constitutively expressed molecular chaperone involved in protein metabolism. In this paper, turbot Hsc70 was cloned and the expression profile was also analyzed. The full-length cDNA of the turbot Hsc70 was 2,292 bp in length, including a 113-bp 5' UTR, a 223-bp 3' UTR and a 1,956-bp open reading frame coding a protein with 651 amino acid residues. Comparison of amino acid sequence revealed the existence of three classical HSP70 family signature motifs, a signature nonapeptide and one repeat of tetrapeptide in turbot Hsc70. The turbot Hsc70-deduced amino acids sequence exhibited 75.4-96.8 % homology with Hsp70s/Hsc70s of 24 other known sequences. In particular, the strongest homology was found with the cognate members of Hsc70 subfamily and the highest identity was found with Japanese flounder Hsc70. Semi-quantitative RT-PCR revealed that turbot Hsc70 transcripts were stably expressed in all tested tissues under normal physiological condition, while the expression levels also increased (similar to 1.5-fold to similar to threefold) after heat shock and bacterial infection. In addition, Hsc70 transcripts were detected throughout embryonic development and in turbot embryonic cell line (TEC) in the absence of any stress. Meanwhile, it was also heat inducible, but not cold inducible in TEC. These results suggest that Hsc70 gene may be involved in embryogenesis and cellular protection events under normal and stress condition.

分类号： S9

  • 相关文献

[1]Comparative analysis of sequence feature and expression of two heat shock cognate 70 genes in mandarin fish Siniperca chuatsi. Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei,Xu, Peng,Zeng, Shuang,Zhou, Lei,Zeng, Lei,Li, Guifeng,Wang, Pengfei.

[2]Molecular cloning and expression of a heat-shock cognate 70 (hsc70) gene from swordtail fish (Xiphophorus helleri). Li Ningqiu,Fu Xiaozhe,Han Jingang,Shi Cunbin,Huang Zhibin,Wu Shuqin. 2013

[3]Identification of heat shock cognate protein 70 gene (Alhsc70) of Apolygus lucorum and its expression in response to different temperature and pesticide stresses. Zhao, Jing,Sheng, Yang,Bai, Li-Xin,Tan, Yongan,Xiao, Liu-Bin,Xu, Guang-Chun,Sun, Yang,Zhang, Yong-Jun,Xiao, Ying-Fang. 2016

[4]Identification and expression analysis of goose-type lysozyme in half-smooth tongue sole (Cynoglossus semilaevis). Sha, Zhen-Xia,Wang, Qi-Long,Chen, Song-Lin,Liu, Yang. 2012

[5]Genome-wide characterization of Toll-like receptor gene family in common carp (Cyprinus carpio) and their involvement in host immune response to Aeromonas hydrophila infection. Gong, Yiwen,Feng, Shuaisheng,Li, Shangqi,Zhang, Yan,Zhao, Zixia,Jiang, Yanliang,Gong, Yiwen,Hu, Mou,Jiang, Yanliang,Xu, Peng. 2017

[6]Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities. Xiang, Jinsong,Li, Xihong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Zhang, Wenting,Zeng, Yan,Sun, Luming,Chen, Song Lin,Sha, Zhenxia,Xiang, Jinsong,Chen, Xuejie,Xiang, Jinsong,Chen, Yadong,Lu, Yang,Yu, Mengjun,Chen, Xuejie,Sha, Zhenxia,Li, Xihong,Chen, Song Lin,Yu, Mengjun,Zhang, Wenting,Zeng, Yan,Sun, Luming.

[7]beta-1,3-Glucan recognition protein 3 activates the prophenoloxidase system in response to bacterial infection in Ostrinia furnacalis Guenee. Zhao, Ya,Xu, Qiuwen,Wang, Yingjuan,Wang, Libao,Zhang, Yiqiang,Feng, Congjing,Wang, Zhenying,Wang, Zengxia,Song, Qisheng. 2018

[8]Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv. oryzae and nitrogen deficiency. Chen Hua-min,Tian Fang,He Chen-Yang,Bi Yong-Mei,Steven, Rothstein J.,Steven, Rothstein J.,Jan, Leach E.. 2015

[9]Molecular cloning, structure and expressional profiles of two novel single-exon genes (PoCCR6A and PoCCR6B) in the Japanese flounder (Paralichthys olivaceus). Wang, Lei,Zhang, Yong-zhen,Xu, Wen-teng,Jia, Xiao-dong,Chen, Song-lin.

[10]Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Lao, Haihua,Bai, Junjie,Yu, Lingyun,Dong, Haiyan,Dong, Haiyan.

[11]Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti. Zhu, Jieyao,Wang, Hong,Zhang, Xin,Hao, Jin,Wu, Yuanbin,Li, Zhiqiong,Chen, Defang,Geng, Yi,Wang, Kaiyu,Zhou, Jian,Chen, Defang,Zhou, Jian,Chen, Defang.

[12]Molecular characterization and quantification of estrogen receptors in turbot (Scophthalmus maximus). Hu, Peng,Meng, Zhen,Jia, Yudong. 2018

[13]Biochemical composition and quality of turbot (Scophthalmus maximus) eggs throughout the reproductive season. Jia, Yudong,Meng, Zhen,Liu, Xinfu,Lei, Jilin,Jia, Yudong,Meng, Zhen,Liu, Xinfu,Lei, Jilin. 2014

[14]Molecular components related to egg quality during the reproductive season of turbot (Scophthalmus maximus). Jia, Yudong,Meng, Zhen,Liu, Xinfu,Lei, Jilin.

[15]Molecular cloning and multifunctional characterization of GRIM-19 (gene associated with retinoid-interferon-induced mortality 19) homologue from turbot (Scophthalmus maximus). Wang, Na,Wang, Tianzi,Chen, Songlin,Wang, Xianli,Yang, Changgeng,Zhao, Xiaojie,Zhang, Yuxi.

[16]Changes in protein composition of epidermal mucus in turbot Scophthalmus maximus (L.) under high water temperature. Ma Ai-Jun,Huang Zhi-hui,Wang Xin-An.

[17]Molecular characterization and quantification of the follicle-stimulating hormone receptor in turbot (Scophthalmus maximus). Jia, Yudong,Sun, Ai,Meng, Zhen,Liu, Baoliang,Lei, Jilin,Jia, Yudong,Sun, Ai,Meng, Zhen,Liu, Baoliang,Lei, Jilin.

[18]Molecular cloning, subcelluar location and expression profile of signal transducer and activator of transcription 2 (STAT2) from turbot, Scophthalmus maximus. Wang, Na,Chen, Song-Lin,Wang, Xian-Li,Yang, Chang-Geng.

[19]Interaction of temperature and salinity on the expression of immunity factors in different tissues of juvenile turbot Scophthalmus maximus based on response surface methodology. Huang Zhihui,Huang Zhihui,Ma Aijun,Wang Xin'an,Lei Jilin,Li Weiye,Wang Ting,Yang Zhi,Qu Jiangbo. 2015

[20]MHC polymorphism and disease-resistance to Edwardsiella tarda in six turbot (Scophthalmus maximus) families. Du Min,Chen SongLin,Yang JingFeng,Zhang Bo,Du Min,Liu YanHong,Niu BaoZhen,Du Min. 2012

作者其他论文 更多>>

微信小程序