Identification of a linear B-Cell epitope on avian reovirus protein sigmaC

文献类型： 外文期刊

第一作者： Yin, Chun-hong

作者： Yin, Chun-hong;Qin, Li-ting;Sun, Mei-yu;Gao, Yu-long;Qi, Xiao-le;Gao, Hong-lei;Wang, Yong-qiang;Jang, Li-li;Wang, Xiao-mei

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关键词： Avian reovirus;Cross reactivity analysis;Linear B-cell epitope;SigmaC protein

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

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收录情况： SCI

摘要： SigmaC (σC) protein, which mediates virus attachment to target cells, is the most variable proteins of avian reovirus (ARV). It is responsible for inducing protective antibody immune responses in animals. To understand the antigenic determinants of σC protein, a set of partially overlapping and consecutive peptides spanning σC were expressed and then screened with the monoclonal antibody (mAb) 2B5 directed against σC. The mAb 2B5 recognized peptides with the σC motif 45ELLHRSISDISTTV58. Further identification of the displayed B-cell epitope was conducted with a set of truncated peptides expressed as GST fusion proteins. The Western blot and ELISA results indicated that 45ELLHRSISDI54 was the minimal determinant of the linear B-cell epitope. Using sequences analysis, we found that this epitope was not a common motif shared among the other members of the ARV and DRV groups. Furthermore, cross reactivity analysis showed that the associated coding motif of other ARV and DRV groups was not recognized by 2B5. These data suggested that 45ELLHRSISDI54 was a type-specific linear B-cell epitope of avian reovirus. The results in this study may have potential applications in the development of diagnostic techniques and epitope-based marker vaccines against ARV, which is prevalent in China.

分类号： R37

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