Characterization of OsMIK in a rice mutant with reduced phytate content reveals an insertion of a rearranged retrotransposon

文献类型： 外文期刊

第一作者： Zhao, Hai-Jun

作者： Zhao, Hai-Jun;Cui, Hai-Rui;Tan, Yuan-Yuan;Fu, Jun-Jie;Shu, Qing-Yao;Zhao, Hai-Jun;Cui, Hai-Rui;Tan, Yuan-Yuan;Fu, Jun-Jie;Shu, Qing-Yao;Xu, Xiu-Hong;Liu, Guo-Zhen;Poirier, Yves

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The rice low phytic acid (lpa) mutant Os-lpa-XS110-1(XS-lpa) has similar to 45 % reduction in seed phytic acid (PA) compared with the wild-type cultivar Xiushui 110. Previously, a single recessive gene mutation was shown to be responsible for the lpa phenotype and was mapped to a region of chromosome 3 near OsMIK (LOC_Os03g52760) and OsIPK1 (LOC_Os03g51610), two genes involved in PA biosynthesis. Here, we report the identification of a large insert in the intron of OsMIK in the XS-lpa mutant. Sequencing of fragments amplified through TAIL-PCRs revealed that the insert was a derivative of the LINE retrotransposon gene LOC_Os03g56910. Further analyses revealed the following characteristics of the insert and its impacts: (1) the inserted sequence of LOC_Os03g56910 was split at its third exon and rejoined inversely, with its 5' and 3' flanking sequences inward and the split third exon segments outward; (2) the LOC_Os03g56910 remained in its original locus in XS-lpa, and the insertion probably resulted from homologous recombination repair of a DNA double strand break; (3) while the OsMIK transcripts of XS-lpa and Xiushui 110 were identical, substantial reductions of the transcript abundance (similar to 87 %) and the protein level (similar to 60 %) were observed in XS-lpa, probably due to increased methylation in its promoter region. The above findings are discussed in the context of plant mutagenesis, epigenetics and lpa breeding.

分类号： R394

  • 相关文献
作者其他论文 更多>>
  • Fungal diversity notes 1919-2016: taxonomic and phylogenetic contributions to fungal taxa

    作者：Cao, Bin;Wang, Long;Li, Jia-Xin;Han, Xi-Xi;Yang, Wen-Qiang;Wang, Shi-Hui;He, Mao-Qiang;Zhao, Rui-Lin;Cao, Bin;Phurbu, Dorji;Zhao, Rui-Lin;Ralaiveloarisoa, Anna;Liimatainen, Kare;Niskanen, Tuula;Liimatainen, Kare;Niskanen, Tuula;Ramirez-Cruz, Virginia;Cortes-Perez, Alonso;Guzman-Davalos, Laura;Castro-Jauregui, Oscar;Bradshaw, Alexander James;Dentinger, Bryn T. M.;Dentinger, Bryn T. M.;Ramirez-Guillen, Florencia;Villalobos-Arambula, Alma Rosa;da Silva, Paula Santos;Day, Rory;Davoodian, Naveed;Lebel, Teresa;May, Tom W.;Lebel, Teresa;Castellano, Michael;Toome, Merje;Vasey, Jack;Hofer, Katharina;Thangavel, Rajaram;Braithwaite, Mark;Braithwaite, Lewis;Dutta, Arun Kumar;Chattopadhyay, Pinaki;Roy, Niranjan;Tanti, Bhaben;Biswas, Pinky Rani;Roy, Niranjan;Arumugam, Elangovan;Kezo, Kezhocuyi;Kaliyaperumal, Malarvizhi;Murugadoss, Ramesh;Ji, Jing-Xin;Kakishima, Makoto;Cooper, Jerry;Nuytinck, Jorinde;Lagaet, Phaedra;De Lange, Ruben;Verbeken, Annemieke;Tondeleir, Lowie;Nuytinck, Jorinde;Luo, Zong-Long;Wang, Wen-Peng;Zhang, Xian;Li, Hua;Xiong, Yin-Ru;Wu, Na;Shu, Yong-Xin;Zhao, Hai-Jun;Liao, Chun-Fang;Yang, Hong-De;Jayawardena, Ruvishika Shehali;Thongklang, Naritsada;Han, Xi-Xi;Hyde, Kevin D.;Zhang, Xian;Wen, Ting-Chi;Zhang, Xian;Wen, Ting-Chi;Jia, Ao-Li;Fan, Xin-Lei;Jiang, Ning;Jiao, Ning;Zhang, Ying;Manawasinghe, Ishara S.;Li, Hua;Xiong, Yin-Ru;Zhou, Tao;Tan, Qing-Ling;Li, Hua;Xiong, Yin-Ru;Wu, Na;Shu, Yong-Xin;Zhao, Hai-Jun;Liao, Chun-Fang;Yang, Hong-De;Jayawardena, Ruvishika Shehali;Thongklang, Naritsada;Han, Xi-Xi;Hoshino, Tamotsu;Hoshino, Tamotsu;Manz, Cathrin;Hampe, Felix;Zhao, Chang-Lin;Yang, Yang;Zhang, Gui-Qing;Dai, Dong-Qin;Tomsovsky, Michal;Denchev, Teodor T.;Denchev, Cvetomir M.;Denchev, Teodor T.;Denchev, Cvetomir M.;Kemler, Martin;Leveille-Bourret, Etienne;Kemler, Martin;Chen, Yan-Peng;Maharachchikumbura, Sajeewa S. N.;Liu, Jian-Kui;Du, Hong-Zhi;Wu, Na;Feng, Zi-Xuan;Wang, Qi-Ming;Wan, Shan-Ping;Yu, Fu-Qiang;Yu, Fu-Qiang;Du, Hong-Zhi;Du, Hong-Zhi;Hu, Hong-Li;Su, Ji-Yu;Wang, Zong-Hua;Hu, Yan-Ping;Yu, Hao;Wang, Zong-Hua;Wang, Jing;Yang, Yi-Hua;Dong, Wei;Shu, Yong-Xin;Zhao, Hai-Jun;Liao, Chun-Fang;Doilom, Mingkwan;Wang, Chao-Qun;Xia, Wen-Xiao;Li, Guo-Jie;Suwannarach, Nakarin;Senwanna, Chanokned;Suwannarach, Nakarin;Senwanna, Chanokned;Gafforov, Yusufjon;Gafforov, Yusufjon;Flakus, Adam;Suchan, Tomasz;Rodriguez-Flakus, Pamela;Plata, Oscar;Tamang, Juna;Acharya, Krishnendu;Tiendrebeogo, Assiata;Somda, Irenee;Decock, Cony;Tiendrebeogo, Assiata;Legreve, Anne;Yang, Yu;Xiao, Yuan-Pin;Zhou, Xian-Zhi;Li, Jia-Xin;Zhao, Rui-Lin;Wang, Shi-Hui;He, Mao-Qiang

    关键词：Ascomycota; Basidiomycota; New combination; New genus; New name; New record; New species

  • Fungal diversity notes 1611-1716: taxonomic and phylogenetic contributions on fungal genera and species emphasis in south China

    作者：Senanayake, Indunil C.;Fu, Jizhen;Luo, Mei;Zhao, Min-Ping;Kularathnage, Nuwan D.;Hua, Li;Yang, Yun-Hui;Liao, Chun-Fang;Zhao, Hai-Jun;Doilom, Mingkwan;Manawasinghe, Ishara S.;Xu, Biao;Hyde, Kevin D.;Song, Jiage;Senanayake, Indunil C.;Du, Tian-Ye;Lu, Li;Kularathnage, Nuwan D.;Hua, Li;Yang, Yun-Hui;Liao, Chun-Fang;Zhao, Hai-Jun;Lestari, Anis S.;Yu, Feng-Ming;Lei, Lei;Liu, Jian-Wei;Karimi, Omid;Sun, Ya-Ru;Zeng, Ming;Htet, Zin H.;Thiyagaraja, Vinodhini;Gomdola, Deecksha;Rathnayaka, Achala R.;Hyde, Kevin D.;Senanayake, Indunil C.;Hongsanan, Sinang;Xie, Ning;Rossi, Walter;Leonardi, Marco;Weir, Alex;McHugh, Mark;Rajeshkumar, Kunhiraman C.;Ashtekar, Nikhil;Singh, Aishwarya;Verma, Rajnish K.;Karunarathna, Samantha C.;Tibpromma, Saowaluck;Du, Tian-Ye;Lu, Li;Jayasiri, Subashini C.;Wijayawardene, Nalin N.;Ashtamoorthy, Sreejith K.;Raveendran, Sanjay;Kour, Gurmeet;De la Pena-Lastra, Saul;Mateos, Antonio;Kolarik, Miroslav;Antonin, Vladimir;Sevcikova, Hana;Esteve-Raventos, Fernando;Moreno, Gabriel;Altes, Alberto;Turegano, Yolanda;Larsson, Ellen;Larsson, Ellen;Pancorbo, Fermin;Li, Qi-Rui;Kang, Ji-Chuan;Dissanayake, Lakmali S.;Gunaseelan, Sugantha;Kezo, Kezhocuyi;Kaliyaperumal, Malarvizhi;Samarakoon, Milan C.;Thiyagaraja, Vinodhini;Gafforov, Yusufjon;Gafforov, Yusufjon;Gafforov, Yusufjon;Teshaboeva, Shakhnoza;Gafforov, Yusufjon;Raza, Mubashar;Kunjan, Pradeep C.;Chamaparambath, Arya;Flakus, Adam;Rodriguez-Flakus, Pamela;Etayo, Javier;Zhurbenko, Mikhail P.;Fedosova, Anna G.;de Silva, Nimali I.;Tennakoon, Danushka S.;Suwannarach, Nakarin;Hongsanan, Sinang;Lumyong, Saisamorn;Hyde, Kevin D.;de Silva, Nimali I.;Tennakoon, Danushka S.;Suwannarach, Nakarin;Hongsanan, Sinang;Phookamsak, Rungtiwa;Lumyong, Saisamorn;Latha, K. P. Deepna;Manimohan, Patinjareveettil;Raj, K. N. Anil;Calabon, Mark S.;Ahmadpour, Abdollah;Heidarian, Zeinab;Alavi, Zahra;Alavi, Fatemeh;Ghosta, Youbert;Azizi, Razmig;Lestari, Anis S.;Yu, Feng-Ming;Lei, Lei;Liu, Jian-Wei;Tang, Song-Ming;Sun, Ya-Ru;Zeng, Ming;Htet, Zin H.;Gomdola, Deecksha;Rathnayaka, Achala R.;Hyde, Kevin D.;Lestari, Anis S.;Yu, Feng-Ming;Lei, Lei;Zeng, Ming;Zhao, Qi;Liu, Jian-Wei;Tang, Song-Ming;Sun, Ya-Ru;Wang, Yong;Linaldeddu, Benedetto T.;Bregant, Carlo;Montecchio, Lucio;Alves, Artur;Phillips, Alan J. L.;De Kesel, Andre;Hustad, Vincent P.;Miller, Andrew N.;Raza, Mubashar;Kucera, Viktor;Hussain, Muzammil;Chen, Yan-Peng;Dissanayake, Asha J.;Maharachchikumbura, Sajeewa S. N.;Liu, Jian-Kui (Jack);Phookamsak, Rungtiwa;Phookamsak, Rungtiwa;Wanasinghe, Dhanushka N.;Phookamsak, Rungtiwa;Jones, E. B. Gareth;Yapa, Neelamanie

    关键词：Agaricomycetes; Ascomycota; Basidiomycota; Dothideomycetes; Eurotiomycetes; Fungal diversity; Geoglossomycetes; Geographical distribution; Host records; Incertae sedis; Laboulbeniomycetes; Lecanoromycetes; Leotiomycetes; Novel taxa; New combination; Oomycota; Peronosporea; Pezizomycetes; Phylogeny; Sordariomycetes; South Chinese fungi; Taxonomy

  • Characterization of an RNase Z nonsense mutation identified exclusively in environment-conditioned genic male sterile rice

    作者：Zhang, Hua-Li;Huang, Jian-Zhong;Nawaz, Zarqa;Lu, Hai-Ping;Shu, Qing-Yao;Zhang, Hua-Li;Huang, Jian-Zhong;Nawaz, Zarqa;Lu, Hai-Ping;Shu, Qing-Yao;Zhang, Hua-Li;Huang, Jian-Zhong;Nawaz, Zarqa;Lu, Hai-Ping;Shu, Qing-Yao;Zhang, Hua-Li;Lu, Hai-Ping;Shu, Qing-Yao;Liu, Qing-Long;Gong, Jun-Yi;Zhu, Yu-Jun;Yan, Wengui

    关键词：Hybrid rice;Gene sequencing;Molecular marker;Plant breeding;Rice

  • Workable male sterility systems for hybrid rice: Genetics, biochemistry, molecular biology, and utilization

    作者：Huang, Jian-Zhong;Zhang, Hua-Li;Shu, Qing-Yao;E, Zhi-Guo

    关键词：Cytoplasmic male sterility (CMS);Environment-conditioned genic male sterility (EGMS);Restorer of fertility (Rf);Chimeric mitochondrial gene;Cytotoxic protein;Pentatricopeptide repeat (PPR)

  • Gene families of maize glutathione-ascorbate redox cycle respond differently to abiotic stresses

    作者：Liu, Yun-Jun;Liu, Yan-Yan;Liu, Yan;Fu, Jun-Jie;Zheng, Jun;Wang, Guo-Ying;Yuan, Yuan

    关键词：Maize;GSH-ASC cycle;Antioxidant;Stress

  • Identification of glutinous maize landraces and inbred lines with altered transcription of waxy gene

    作者：Bao, Jian-dong;Hu, Wei-min;Fan, Long-jiang;Bao, Jian-dong;Yao, Jian-qiang;Zhu, Jin-qing;Cai, Da-guang;Li, Yu;Shu, Qing-yao

    关键词：Granule-bound starch synthase;Wx mutation;Alternative splicing;Chinese waxy maize

  • Identification and characterization of the soybean IPK1 ortholog of a low phytic acid mutant reveals an exon-excluding splice-site mutation

    作者：Tan, Yuan-Yuan;Shu, Qing-Yao;Tan, Yuan-Yuan;Shu, Qing-Yao;Yuan, Feng-Jie;Zhu, Dan-Hua;Dong, De-Kun;Fu, Xu-Jun;Zhu, Shen-Long;Li, Bai-Quan

    关键词：

微信小程序