Composition analysis and anti-proliferation activity of polysaccharides from Dendrobium chrysotoxum

文献类型: 外文期刊

第一作者: Sun, Yan-Dong

作者: Sun, Yan-Dong;Wang, Zai-Hua;Ye, Qing-Sheng;Wang, Zai-Hua

作者机构:

关键词: Dendrobium chrysotoxum;Polysaccharide;Anti-proliferation activity

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )

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收录情况: SCI

摘要: Water-soluble polysaccharides (DCCP,DCPP) were extracted from the stems of Dendrobium chrysotoxum using boiling water and ultrasound. DCPP were successively purified by chromatography on DEAE-Cellulose-52 and Sephadex G-200 column,giving three major polysaccharide fractions termed DCPP-I,DCPP-I-a and DCPP-II. Among these fractions,DCPP-I-a exhibited the highest abundance (79.5%). The number-average molecular weight and weight-average molecular weight of DCPP-I-a were 67 kDa and 122 kDa,respectively. Monosaccharide components analysis indicated that DCPP-I-a were composed of xylose,glucose,galactose in a molar ratio of 1.44:6.93:12.79. Infrared spectra (IR) and ~1H NMR showed that DCPP-I-a was composed of a β-D pyran ring with a β-configuration. The evaluation of anti-proliferation activity suggested that DCPP-I-a and DCPP-II had a higher inhibitory effect on the SPC-A-1 cells than DCCP as determined with in vitro anti-proliferation test.

分类号: Q71

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