Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder

文献类型: 外文期刊

第一作者: Liu, Yi

作者: Liu, Yi;Zhang, Xiaoyan;Si, Fei;Sun, Zhaohui;Wang, Guixing;Wang, Yufen;Liu, Yi;Liu, Yongxin;Liu, Yingjie;Yang, Runqing;Liu, Haijin

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关键词: doubled haploid;linkage map;type II SSR;EST-SSR;Japanese flounder;skeletal traits

期刊名称:BIOLOGIA ( 影响因子:1.35; 五年影响因子:1.139 )

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收录情况: SCI

摘要: A genetic linkage map of Japanese flounder was constructed using 165 doubled haploids (DHs) derived from a single female. A total of 574 genomic microsatellites (type II SSRs) and expressed sequence tag (EST)-derived markers (EST-SSRs) were mapped to 24 linkage groups. The length of linkage map was estimated as 1270.9 centiMorgans (cM), with an average distance between markers of 2.2 cM. The EST-SSRs were used together with type II SSR markers to construct the Japanese flounder genetic linkage map which will facilitate identify quantitative trait locus (QTL) controlling important economic traits in Japanese flounder. Thus, twelve skeletal traits at 2 years of age were measured for all DHs. Forty-one QTLs were detected on 14 linkage groups and totally account for a small proportion of phenotypic variation (4.5 to 17.3%). Most of QTLs detected distribute on linkage groups 5 (9 QTLs), 8 (9 QTLs), 9 (5 QTLs) and 20 (4 QTLs), in which, some QTLs perform the pleiotropy.

分类号: Q

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