Mutation Breeding of Lipase-producing Strain Flavobacterium sp by Supercritical CO2 with Hydrazine Hydrate

文献类型： 外文期刊

第一作者： Zhang, Qiaoyan

作者： Zhang, Qiaoyan;Qian, Junqing;Ma, Lingzhi;Zhang, Qiaoyan

作者机构：

关键词： Supercritical CO2;Mutation;pH Drop;Lipase;Flavobacterium sp

期刊名称：BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY （ 影响因子：0.797； 五年影响因子：1.193 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This work aimed to obtain an ideal mutant strain with higher lipase yield using hydrazine hydrate (HZH) as a novel additive to treat Flavobacterium sp. strain YY25 by supercritical CO2. The survival rate and the positive mutation rate of the tested strain were strongly dependent on the dose of HZH. The treatment by 0.5% HZH in supercritical CO2 (8 MPa, 35 degrees C) for 30 min provided 58.3% of positive mutation rate and an expected mutant strain with about 76.7% increase in lipase yield compared with the wild strain. Possible mutagenesis mechanisms were further explored. The analysis on pH drop of the treated seed liquid was performed to better understand the interaction. Results proved that the induced mutation with enhanced yield of lipase could be achieved by co-mutagenesis of supercritical CO2 and HZH.

分类号： Q7

  • 相关文献

[1]Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation. Tian, Jian,Wang, Ping,Gao, Shan,Chu, Xiaoyu,Wu, Ningfeng,Fan, Yunliu.

[2]Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.). Liu, Qing-Long,Xu, Xiu-Hong,Ren, Xue-Liang,Fu, Hao-Wei,Wu, Dian-Xing,Shu, Qing-Yao.

[3]Study on the dynamic surface tension of nonionic surfactant LS45 and LS54 solutions. Wang, Suqing,Zheng, Liqiang,Li, Ganzuo,Hou, Wanguo,Zhang, Xuan,Lu, Deming,Zhang, Shaohua,Zhang, Gaoyong,Huang, Qiliang.

[4]Supercritical CO2: A novel environmentally friendly mutagen. Zhang, Qiao Y.,Qian, Jun Q.,Guo, Hui,Yang, Sheng L.,Zhang, Qiao Y.. 2008

[5]Effects of DIMBOA on several enzymatic systems in Asian corn borer, Ostrinia furnacalis (Guenee). Yan, FM,Xu, CG,Li, SG,Lin, CS,Li, JH. 1995

[6]Isolation and biochemical characterization of two lipases from a metagenomic library of China Holstein cow rumen. Liu, Kailang,Wang, Jiaqi,Bu, Dengpan,Zhao, Shengguo,Yu, Ping,Li, Dan,McSweeney, Chris. 2009

[7]Progress of Lipase-Catalyzed Ester Synthesis in Ionic Liquid. Li Jing,Wang Jun,Zhang Leixia,Gu Shuangshuang,Wu Fuan,Guo Yuewei,Wu Fuan,Guo Yuewei. 2012

[8]A novel chemoenzymatic synthesis of propyl caffeate using lipase-catalyzed transesterification in ionic liquid. Pang, Na,Gu, Shuang-Shuang,Wang, Jun,Cui, Hong-Sheng,Wang, Fang-Qin,Liu, Xi,Zhao, Xing-Yu,Wu, Fu-An,Wu, Fu-An.

[9]Pretreating porcine sperm with lipase enhances developmental competence of embryos produced by intracytoplasmic sperm injection. Wei, Yinghui,Fan, Junhua,Li, Lin,Liu, Zhiguo,Li, Kui.

[10]A novel proteolysis-resistant lipase from keratinolytic Streptomyces fradiae var. k11. Zhang, Yuhong,Meng, Kun,Wang, Yaru,Luo, Huiying,Yang, Peilong,Shi, Pengjun,Yao, Bin,Wu, Ningfeng,Fan, Yunhu,Li, Jiang.

[11]Synthesis and Characterization of Magnetic Nanoparticles and Its Application in Lipase Immobilization. Xu, Jiakun,Ju, Caixia,Sheng, Jun,Wang, Fang,Zhang, Quan,Sun, Guolong,Sun, Mi,Xu, Jiakun,Ju, Caixia,Zhang, Quan,Sun, Guolong. 2013

[12]Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquids: Effect of Specific Ions and Reaction Parameters. Wang Jun,Li Jing,Zhang Leixia,Gu Shuangshuang,Wu Fuan,Wu Fuan. 2013

[13]Lipase-Catalyzed Acyl-L-Carnitines Synthesis in [Bmim]PF6 Ionic Liquid. Tian, Jin-qiang,Wang, Qiang. 2009

[14]Lipase-catalyzed acylation of l-carnitine with conjugated linoleic acid in [Bmim]PF6 ionic liquid. Tian, Jinqiang,Wang, Qiang,Tian, Jinqiang,Wang, Qiang,Zhang, Zhongyuan. 2009

[15]Lipase Diversity in Glacier Soil Based on Analysis of Metagenomic DNA Fragments and Cell Culture. Zhang Yuhong,Shi, Pengjun,Meng, Kun,Bai, Yingguo,Wang, Guozeng,Yao, Bin,Zhang Yuhong,Liu, Wanli,Zhan, Zhichun.

[16]Ultrasound irradiation accelerates the lipase-catalyzed synthesis of methyl caffeate in an ionic liquid. Wang, Jun,Wang, Shasha,Li, Zhongjian,Gu, Shuangshuang,Wu, Fuan,Wang, Jun,Wang, Shasha,Li, Zhongjian,Gu, Shuangshuang,Wu, Fuan,Wang, Jun,Wu, Xiangyang.

[17]From microalgae oil to produce novel structured triacylglycerols enriched with unsaturated fatty acids. Wang, Jun,Wang, Xu-Dong,Zhao, Xing-Yu,Liu, Xi,Wu, Fu-An,Wang, Jun,Wu, Fu-An,Dong, Tao.

[18]Isolation of a Novel Lipase Gene from Serratia liquefaciens S33 DB-1, Functional Expression in Pichia pastoris and its Properties. Yao, Hongyan,Yu, Shunwu,Zuo, Kaijing,Ling, Hua,Zhang, Fei,Tang, Kexuan,Yu, Shunwu.

[19]Physicochemical properties of lipase-catalyzed laurylation of corn starch. Gao, Yan,Wang, Lan,Xiong, Guangquan,Wu, Wenjin,Qiao, Yu,Liao, Li,Gao, Yan,Yue, Xiali. 2014

[20]Microfluidic biocatalysis enhances the esterification of caffeic acid and methanol under continuous-flow conditions. Wang, Sha-Sha,Li, Zhong-Jian,Sheng, Sheng,Wu, Fu-An,Wang, Jun,Sheng, Sheng,Wu, Fu-An,Wang, Jun.

作者其他论文 更多>>

微信小程序