Characterization of Dicrocoelium chinensis from domestic yaks in Gansu and Sichuan provinces, China, using genetic markers in two mitochondrial genes

文献类型： 外文期刊

第一作者： Wang, Xing-ye

作者： Wang, Xing-ye;Zhao, Guang-hui;Lin, Qing;Wang, Xing-ye;Zhao, Guang-hui;Liu, Guo-hua;Li, Jia-yuan;Zhou, Dong-hui;Xu, Min-jun;Lin, Qing;Zhu, Xing-quan;Liu, Guo-hua;Zhu, Xing-quan;Li, Jia-yuan;Zhu, Xing-quan

作者机构：

关键词： Dicrocoelium chinensis;mitochondrial DNA (mtDNA);sequence variation;cytochrome c oxidase subunit 1 (coxl);NADH dehydrogenase subunit 1 (nadl);phylogenetic analysis

期刊名称：MITOCHONDRIAL DNA （ 影响因子：0.925； 五年影响因子：0.994 ）

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收录情况： SCI

摘要： Dicrocoelium chinensis is commonly known as the "lancet fluke" or "small liver fluke" which lives in the bile ducts and gall bladder of domestic and wild ruminants. In this study, we examined sequence variability in two mitochondrial (mt) genes, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nadl) genes, among D. chinensis isolated from domestic yaks in Gansu and Sichuan provinces, China. A portion of the cox1 (pcoxl) and nadl (pnadl) genes was amplified by PCR separately from the individual adult D. chinensis, and the amplicons were subjected to sequencing from both directions. The lengths of the sequences of pcoxl and pnadl were 358 and 458 bp, respectively. The A + T contents of the sequences were 58. 94 - 59. 22% for pcoxl and 60. 04 - 60. 48% for pnadl, respectively. The intra-specific sequence variations within D. chinensis were 0-0.84% for pcoxl and 0-0.87% for pnadl. The inter-specific sequence differences among members of the examined trematodes were significantly higher, being 22.9-29.3% and 33.6-41.5% for pcoxl and pnadl, respectively. Phylogenetic analysis utilizing combined sequences of pcoxl and pnadl using Bayesian inference revealed that all of the D. chinensis isolates grouped together with high statistical support. These findings demonstrated the existence of low intra- specific variation in mitochondrial DNA (mtDNA) sequences among the D. chinensis isolates from domestic yaks in China and had implications for studying the molecular epidemiology and population genetics of D. chinensis.

分类号： Q81

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