Activation of Bombyx neuropeptide G protein-coupled receptor A4 via a G alpha i-dependent signaling pathway by direct interaction with neuropeptide F from silkworm, Bombyx mori

文献类型： 外文期刊

第一作者： Deng, Xiaoyan

作者： Deng, Xiaoyan;Yang, Huipeng;He, Xiaobai;Liao, Yuan;Zheng, Congxia;Zhu, Chenggang;Zhou, Naiming;Zhang, Guozheng;Zhou, Qi;Gao, Jimin

作者机构：

关键词： Neuropeptide;GPCR;Signaling;Arrestin;MAP kinases

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.714； 五年影响因子：4.953 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Members of the mammalian neuropeptide Y (NPY) family serve as neurotransmitters and contribute to a diversity of physiological functions. Although neuropeptide F (NPF), the NPY-like orthologs from insects, have been identified, the NPF receptors and their signaling and physiological functions remain largely unknown. In this study, we established the stable and transient functional expression of a Bombyx orphan G protein-coupled receptor, BNGR-A4, in both mammalian HEK293 and insect SF21 cells. We identified Bombyx mori NPFs as specific endogenous ligands for the Bombyx Neuropeptide GPCR A4 (BNGR-A4) and, accordingly, named the receptor BomNPFR. Our results demonstrated that BomNPFR was activated by synthetic BomNPF1a and BomNPF1b at a high efficacy and by BomNPF2 at a low efficacy. This activation led to a decrease of forskolin or adipokinetic hormone peptide-stimulated adenylyl cyclase activity, an increase of intracellular Ca2+, the activation of ERK1/2 signaling and receptor internalization. Moreover, a Rhodamine-labeled BomNPF1a peptide was found to bind specifically to BomNPFR. The results derived from quantitative RT-PCR analysis and dsRNA-mediated knockdown experiments demonstrated the possible role of BomNPFR in the regulation of food intake and growth. Our results provide the first in-depth information on BomNPFR-mediated signaling for the further elucidation of the BomNPF/BomNPFR system in the regulation of fundamental physiological processes.

分类号： Q5

  • 相关文献

[1]Activation of cAMP-response element-binding protein is positively regulated by PKA and calcium-sensitive calcineurin and negatively by PKC in insect. Yang, Huipeng,He, Xiaobai,Deng, Xiaoyan,Liao, Yuan,Zhu, Chenggang,Shi, Ying,Zhou, Naiming,Yang, Jingwen,Zhang, Zhifang.

[2]The G protein-coupled receptors in the silkworm, Bombyx mori. Sun, Peng,Wang, Yu,Chen, Xin,Fan, Yi,He, Xiaobai,Deng, Xiaoyan,Chen, Xiaopan,Zhou, Naiming,Zhang, Guozheng.

[3]Molecular cloning, expression analysis and cellular localization of an LFRFamide gene in the cuttlefish Sepiella japonica. Cao, Zi-hao,Sun, Lian-lian,Chi, Chang-feng,Liu, Hui-hui,Lv, Zhen-ming,Wu, Chang-wen,Zhou, Li-qing.

[4]A Comprehensive Proteomic Survey of ABA-Induced Protein Phosphorylation in Rice (Oryza sativa L.). Qiu, Jiehua,Hou, Yuxuan,Wang, Yifeng,Li, Zhiyong,Zhao, Juan,Tong, Xiaohong,Lin, Haiyan,Wei, Xiangjin,Zhang, Jian,Lin, Haiyan,Ao, Hejun. 2017

[5]Mechanisms and Physiological Roles of the CBL-CIPK Networking System in Arabidopsis thaliana. Mao, Jingjing,Manik, S. M. Nuruzzaman,Shi, Sujuan,Chao, Jiangtao,Wang, Qian,Liu, Haobao,Shi, Sujuan,Jin, Yirong. 2016

[6]A Quantitative Proteomic Analysis of Brassinosteroid-induced Protein Phosphorylation in Rice (Oryza sativa L.). Hou, Yuxuan,Qiu, Jiehua,Wang, Yifeng,Li, Zhiyong,Zhao, Juan,Tong, Xiaohong,Lin, Haiyan,Zhang, Jian,Lin, Haiyan. 2017

[7]alpha-Farnesene and ocimene induce metabolite changes by volatile signaling in neighboring tea (Camellia sinensis) plants. Zeng, Lanting,Liao, Yinyin,Zhou, Ying,Yang, Ziyin,Zeng, Lanting,Liao, Yinyin,Zhou, Ying,Yang, Ziyin,Zeng, Lanting,Liao, Yinyin,Yang, Ziyin,Li, Jianlong,Tang, Jinchi,Li, Jianlong,Tang, Jinchi,Dong, Fang.

[8]Functional analysis of tomato LeEIL1 in an Arabidopsis ein2 mutant. Pan, Yu,Chen, Guoping,Hu, Zongli,Lu, Chungui,Chen, Xuqing.

[9]Nitric oxide production is associated with response to brown planthopper infestation in rice. Liu, Yuqiang,He, Jun,Jiang, Ling,Wu, Han,Xiao, Yuehua,Liu, Yanlin,Li, Guangquan,Du, Yueqiang,Liu, Chenyang,Wan, Jianmin,Wan, Jianmin.

[10]Comparative Transcriptomic Profiling of a Salt-Tolerant Wild Tomato Species and a Salt-Sensitive Tomato Cultivar. Sun, Wei,Zhu, Huishan,Liu, Aihua,Hua, Xuejun,Xu, Xinna,Liu, Lei,Li, Junming.

[11]Comparative Transcriptome Analysis of Primary Roots of Brassica napus Seedlings with Extremely Different Primary Root Lengths Using RNA Sequencing. Dun, Xiaoling,Tao, Zhangsheng,Wang, Jie,Wang, Xinfa,Liu, Guihua,Wang, Hanzhong. 2016

[12]CharacterizationofmousebrainmicroRNAsafterinfectionwithcyst-formingToxoplasmagondii. 徐民俊,DonghuiZhou,AlasdairJ.Nisbet,SiyangHuang,YifanFan,XingquanZhu. 2013

[13]Phytochromes Regulate SA and JA Signaling Pathways in Rice and Are Required for Developmentally Controlled Resistance to Magnaporthe grisea. Xie, Xian-Zhi,Xue, Yan-Jiu,Zhou, Jin-Jun,Zhang, Bin,Xie, Xian-Zhi,Zhou, Jin-Jun,Zhang, Bin,Xue, Yan-Jiu,Chang, Hong,Takano, Makoto.

[14]Exogenous sucrose treatment accelerates postharvest tomato fruit ripening through the influence on its metabolism and enhancing ethylene biosynthesis and signaling. Li, Dongdong,Mou, Wangshu,Li, Li,Mao, Linchun,Ying, Tiejin,Luo, Zisheng,Wang, Yansheng.

[15]Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44. Li, Dekuan,Li, Ying,Zhang, Liang,Wang, Xiaoyu,Zhao, Zhe,Tao, Zhiwen,Wang, Jianmei,Li, Xufeng,Yang, Yi,Li, Dekuan,Yang, Yi,Wang, Jin,Lin, Min. 2014

[16]Proteome analysis of maize seeds: the effect of artificial ageing. Xin, Xia,Lin, Xin-Hai,Chen, Xiao-Ling,Liu, Xu,Lu, Xin-Xiong,Lin, Xin-Hai,Zhou, Yuan-Chang.

[17]A new Akirin1 gene in turbot (Scophthalmus maximus): Molecular cloning, characterization and expression analysis in response to bacterial and viral immunological challenge. Yang, Chang-Geng,Wang, Xian-Li,Wang, Lei,Zhang, Bo,Chen, Song-Lin,Yang, Chang-Geng.

[18]Overexpression of the PP2A-C5 gene confers increased salt tolerance in Arabidopsis thaliana. Hu, Rongbin,Zhu, Yinfeng,Zhang, Hong,Shen, Guoxin.

[19]Genome-wide survey and expression analysis of the calcium-dependent protein kinase gene family in cassava. Hu, Wei,Hou, Xiaowan,Xia, Zhiqiang,Yan, Yan,Wei, Yunxie,Zou, Meiling,Lu, Cheng,Wang, Wenquan,Peng, Ming,Wang, Lianzhe.

作者其他论文 更多>>

微信小程序