Identification and molecular characterization of an Akirin2 homolog in Chinese loach (Paramisgurnus dabryanus)
文献类型: 外文期刊
第一作者: Xue, Xianli
作者: Xue, Xianli;Chen, Yeyu;Zhang, Xinshang;Luo, Huiying;Li, Zhongyuan;Zhao, Heng;Yao, Bin;Wang, Liwen
作者机构:
关键词: Chinese loach (Paramisgurnus dabryanus);Akirin2;Inducible expression;Cytokines;Transcription factor
期刊名称:FISH & SHELLFISH IMMUNOLOGY ( 影响因子:4.581; 五年影响因子:4.851 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Akirin is a nuclear factor involved in innate immune responses of arthropods and mammals. In this study we have cloned an Akirin2 gene, pdakirin2, from freshwater Chinese loach (Paramisgurnus dabryanus) and characterized its biological functions. Phylogenetic analysis revealed deduced PdAkirin2 had high sequence identities to Akirin2 homologs from fish and mammals (70-91%), it contained two conserved nuclear localization signals (NLSs) with verified sub-cellular localization. Quantitative real-time (qRT)-PCR analysis indicated that PdAkirin2 was present in a wide range of loach tissues and showed upregulation with challenges of Aeromonas hydrophila NJ-1, LPS and poly I:C. PdAkirin2 as an immune factor had significant effects on the expression of cytokines (TNF alpha, IFN-alpha, IFN-gamma, IL-4 and IL-1 beta) and transcription factor NF-kappa B. This study provides insights into the potential role of PdAkirin2 in the innate immune system.
分类号: S9
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