Whole blood transcriptome comparison of pigs with extreme production of in vivo dsRNA-induced serum IFN-a

文献类型： 外文期刊

第一作者： Liu, Xiangdong

作者： Liu, Xiangdong;Huang, Jing;Yang, Songbai;Zhao, Yunxia;Xiang, Anjing;Cao, Jianhua;Fan, Bin;Zhao, Shuhong;Zhu, Mengjin;Liu, Xiangdong;Huang, Jing;Yang, Songbai;Zhao, Yunxia;Xiang, Anjing;Cao, Jianhua;Fan, Bin;Zhao, Shuhong;Zhu, Mengjin;Liu, Xiangdong;Zhao, Junlong;Wu, Zhenfang;Huang, Jing

作者机构：

关键词： Pig;dsRNA;Blood;IFN-a;Transcriptome;Poly I:C

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Interferon (IFN) is one of the major regulators of innate immunity, it also mediates the adaptive immune responses to a broad spectrum of pathogens. This study aims in identifying differences between high vs. low INF-a responders which were chosen based on serum INF-a levels at 4 h post poly I:C treatment. A transcriptomic analysis was designed to describe the whole blood differential transcriptomal response to poly I:C by pigs with high vs. low IFN alpha levels. The capability of producing dsRNA (poly I:C)-induced serum IFN-a is highly variable in pig population. The high INF-a responders had 328 unique differentially expressed genes, suggesting that the HIGH pigs have greater responsiveness upon the dsRNA simulation. Based on the results, the interferon-dependent antiviral responsiveness through the IFNstimulated genes (ISGs) is likely more effective in HIGH pigs. Inferring from the known organization of IFN pathways, the reason for the more IFN-a production in the HIGH pigs was likely due to the enhanced expression of IRF-7 in TLR or RIG- I/MDA5 signaling pathways. Furthermore, the larger number of the altered genes in the HIGH pigs after simulation is also possibly because of the greater number of the altered transcription factors. To our knowledge, this is the first report of comparative transcriptomic analysis to advance our understanding of whole blood immune response in pigs with different in vivo poly I:C-inducted IFN-a levels. The paper significantly expands our knowledge of how pigs respond to poly I:C which is highly relevant for understanding resistance to viral infections and also for vaccine development.

分类号： R392

  • 相关文献

[1]Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs. Wang, Yuding,Ma, Cai,Sun, Yi,Kang, Li,Jiang, Yunliang,Wang, Yuding,Li, Yi. 2017

[2]Comparison of skeletal muscle miRNA and mRNA profiles among three pig breeds. Hou, Xinhua,Yang, Yalan,Zhu, Shiyun,Hua, Chaoju,Zhou, Rong,Mu, Yulian,Tang, Zhonglin,Li, Kui,Yang, Yalan,Tang, Zhonglin.

[3]Transcriptome analysis reveals that constant heat stress modifies the metabolism and structure of the porcine longissimus dorsi skeletal muscle. Hao, Yue,Feng, Yuejin,Yang, Peige,Cui, Yanjun,Liu, Jiru,Yang, Chunhe,Gu, Xianhong,Liu, Jiru.

[4]Babesia sp. BQ1 (Lintan): Molecular evidence of experimental transmission to sheep by Haemaphysalis qinghaiensis and Haemaphysalis longicornis. Guan, Guiquan,Moreau, Emmanuelle,Chauvin, Alain,Guan, Guiquan,Liu, Junlong,Hao, Xuefen,Ma, Miling,Luo, Jianxun,Yin, Hong.

[5]MicroRNA Transcriptome of Poly I:C-Stimulated Peripheral Blood Mononuclear Cells Reveals Evidence for MicroRNAs in Regulating Host Response to RNA Viruses in Pigs. Wang, Jiying,Wang, Yanping,Guo, Jianfeng,Wang, Huaizhong,Wu, Ying,Wang, Jiying,Wang, Haifei,Liu, Jianfeng. 2016

[6]A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. Liu, Liang,Guo, Lihua,Qiu, Dewen,Kondo, Hideki.

[7]Mycoreovirus genome alterations: similarities to and differences from rearrangements reported for other reoviruses. Tanaka, Toru,Eusebio-Cope, Ana,Suzuki, Nobuhiro,Sun, Liying. 2012

[8]Activation of the PKR/eIF2 alpha signaling cascade inhibits replication of Newcastle disease virus. Zhang, Shilei,Sun, Yingjie,Chen, Hongjun,Zhan, Yuan,Yu, Shengqing,Qiu, Xusheng,Tan, Lei,Song, Cuiping,Ding, Chan,Dai, Yabin. 2014

[9]dsRNA interference on expression of a RNA-dependent RNA polymerase gene of Bombyx mori cytoplasmic polyhedrosis virus. Pan, Zhong-Hua,Gao, Kun,Hou, Cheng-Xiang,Wu, Ping,Qin, Guang-Xing,Geng, Tao,Guo, Xi-Jie,Pan, Zhong-Hua,Gao, Kun,Hou, Cheng-Xiang,Wu, Ping,Qin, Guang-Xing,Geng, Tao,Guo, Xi-Jie,Pan, Zhong-Hua.

[10]Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum. Lin, Yanhong,Zhang, Hailong,Wang, Shuangchao,Qiu, Dewen,Guo, Lihua.

[11]Transfection of BmCPV genomic dsRNA in silkmoth-derived Bm5 cells: Stability and interactions with the core RNAi machinery. Swevers, Luc,Kolliopoulou, Anna,Li, Zheng,Sun, Jingchen,Daskalaki, Maria,Verret, Frederic,Kalantidis, Kriton,Smagghe, Guy. 2014

[12]Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction. Eusebio-Cope, Ana,Suzuki, Nobuhiro,Sun, Liying,Hillman, Bradley I.. 2010

[13]Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum. Zhang, Hailong,Chen, Xiaoguang,Qiu, Dewen,Guo, Lihua.

[14]A double-stranded RNA degrading enzyme reduces the efficiency of oral RNA interference in migratory locust. Song, Huifang,Zhang, Jianqin,Li, Daqi,Li, Tao,Liu, Xiaojian,Ma, Enbo,Zhang, Jianzhen,Song, Huifang,Zhang, Jianqin,Li, Daqi,Cooper, Anastasia M. W.,Silver, Kristopher,Liu, Xiaojian,Zhu, Kun Yan.

[15]Prevalence and diversity of mycoviruses infecting the plant pathogen Ustilaginoidea virens. Zhang, Tingting,Luo, Chaoxi,Jiang, Daohong,Li, Guoqing,Huang, Junbin,Li, Qili,Hsiang, Tom.

[16]GROWTH PERFORMANCE AND ANTIOXIDANT CAPACITIES IN PIGLETS WITH DIFFERENT WEANING AGE. Li, L. A.,Yang, J. J.,Li, Y.,Jin, T. M.,Li, R. N.,Qin, S. Y.,Xie, J. J.,Du, G. M.. 2016

[17]Oxidative Stress and Antioxidant Defenses after Long-term Fasting in Blood of Chinese Sturgeon (Acipenser sinensis). Feng, Guangpeng,Huang, Xiaorong,Zhuang, Ping,Shi, Xiaotao. 2011

[18]Effects of Temperature on Oxidative Stress Biomarkers in Juvenile Chinese Sturgeon (Acipenser Sinensis) under Laboratory Conditions. Feng, Guangpeng,Zhuang, Ping,Zhang, Longzhen,Duan, Ming,Liu, Jianyi. 2012

[19]Effects of feeding beta-carotene on levels of beta-carotene and vitamin A in blood and tissues of beef cattle and the effects on beef quality. Jin, Qing,Cheng, Haijian,Wan, Fachun,Liu, Guifen,Liu, Xiaomu,Zhao, Hongbo,You, Wei,Liu, Yifan,Tan, Xiuwen,Jin, Qing,Cheng, Haijian,Wan, Fachun,Liu, Guifen,Liu, Xiaomu,Zhao, Hongbo,You, Wei,Liu, Yifan,Tan, Xiuwen,Bi, Yulin.

[20]Experimental infection with bovine ephemeral fever virus and analysis of its antibody response cattle. Zheng, F. Y.,Chen, Q. W.,Li, Z.,Gong, X. W.,Wang, J. D.,Yin, H..

作者其他论文 更多>>

微信小程序