Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek's disease virus

文献类型: 外文期刊

第一作者: Yu, Zu-Hua

作者: Yu, Zu-Hua;Teng, Man;Yu, Le-Le;Hu, Bo;Zhang, Gai-Ping;Luo, Jun;Yu, Zu-Hua;Hu, Bo;Liu, Ju-Xiong;Zhang, Gai-Ping;Sun, Ai-Jun;Cui, Zhi-Zhong;Qu, Liang-Hu;Yu, Zu-Hua;Ding, Ke;Cheng, Xiang-Chao;Luo, Jun

作者机构:

关键词: MDV;MicroRNA;miR-155;mdvl-miR-M4;BAC;Pathogenesis;Oncogenesis

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

ISSN:

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收录情况: SCI

摘要: The microRNA (miRNA) mdvl-miR-M4, a functional miR-155 ortholog encoded by oncogenic Marek's disease virus (MDV), has previously been suggested to be involved in MDV pathogenesis. Using the technique of bacterial artificial chromosome mutagenesis, we have presently evaluated the potential role of mdvl-miR-M4 in the oncogenesis of the very virulent (w) MDV strain GX0101. Unexpectedly, deletions of the Meq-cluster or mdvl-miR-M4 alone from the viral genome strongly decreased rather than abolished its oncogenicity. Compared to GX0101, mortalities of mutants GXAmiR-M4 and GXAMeq-miRs were reduced from 100% to 18% and 4%, coupled with the gross tumor incidence reduction from 28% to 22% and 8%, respectively. Our data suggests that the mdvl-miR-M4 is possibly an important regulator in the development of Marek's disease (MD) lymphomas but is not essential for the oncogenicity of wMDV. In addition, some of the other Meq-clustered miRNAs may also play potentially critical roles in wMDV induction of lymphomas.

分类号: R37

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