The epitope structure of Citrus tristeza virus coat protein mapped by recombinant proteins and monoclonal antibodies

文献类型: 外文期刊

第一作者: Wu, Guan-Wei

作者: Wu, Guan-Wei;Wang, Guo-Ping;Hong, Ni;Tang, Min;Wang, Guo-Ping;Wang, Cai-Xia;Liu, Yong;Yang, Fan;Hong, Ni;Wang, Cai-Xia;Liu, Yong

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关键词: Citrus tristeza virus;Coat protein;Epitope mapping;Recombinant protein;Monoclonal antibody;Structure analysis

期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )

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收录情况: SCI

摘要: It has been known that there exists serological differentiation among Citrus tristeza virus (CTV) isolates. The present study reports three linear epitopes (aa 48-63, 97-104, and 114-125) identified by using bacterially expressed truncated coat proteins and ten monoclonal antibodies against the native virions of CTV-S4. Site-directed mutagenesis analysis demonstrated that the mutation D98G within the newly identified epitope 97DDDSTGIT104 abolished its reaction to MAbs 1, 4, and 10, and the presence of G98 in HB1-CP also resulted in its failure to recognize the three MAbs. Our results suggest that the conformational differences in the epitope I 48LGTO_O_NAALNRDLFLT63 between the CPs of isolates S4 and HB1 might contribute to the different reactions of two isolates to MAbs 5 and 6. This study provides new information for the antigenic structures of CTV, and will extend the understanding of the processes required for antibody binding and aid the development of epitope-based diagnostic tools.

分类号: R37

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