Screening and Cultivation Conditions of a Bacteroides Strain from Tideland Soil

文献类型： 外文期刊

第一作者： Liu, Quanquan

作者： Liu, Quanquan;Zheng, Jingtang;Yang, Lei

作者机构：

关键词： Bacteroides;Tideland Soil;Cellulose;Phylogenetic;Identification

期刊名称：JOURNAL OF PURE AND APPLIED MICROBIOLOGY （ 影响因子：0.073； ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A novel strictly anaerobic, heterotrophic bacterium, strain D-2, was isolated from the tideland soil of East China sea. This organism was identified as a member of the genus Bacteroides on the basis of its 16S rRNA gene sequence. Cells stained Gram-negative, were gram-negative, no-spore, appeared as irregular rod. Cells were 0.5 jum wide and 1-3 /xm long, and occurred in clusters of two to four cells. Its optimum temperature, pH and NaCl concentration for growth were 37 °C, 7.0 and 20 g-L\ respectively. Under the optimum growth conditions, the doubling time was approximately 91.8 min. Strain D-2 fermented a variety of simple and complex substrates such as glucose, cellobiose, ribose, raffinose(2 mM), lactose, xylan, arabinose and so on. The end products identified during growth on glucose were acetate, propionate, butyrate, C02, and H2. The DNA G+C content of this organism was 43.9 mol%. Based on the phenotypic and phylogenetic characteristics, it is proposed that this organism represents a novelspecies in the genus Bacteroides. The Genbank Number is GU384203.

分类号： Q93

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