Identification of a novel vga(E) gene variant that confers resistance to pleuromutilins, lincosamides and streptogramin A antibiotics in staphylococci of porcine origin

文献类型： 外文期刊

第一作者： Li, Jun

作者： Li, Jun;Wang, Yang;Wu, Congming;Shen, Jianzhong;Li, Beibei;Ma, Zhiyong;Wendlandt, Sarah;Schwarz, Stefan

作者机构：

关键词： ABC transporters;Animal staphylococci;Plasmids;Recombination;Transposons

期刊名称：JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY （ 影响因子：5.79； 五年影响因子：5.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Objectives: To investigate the genetic basis of pleuromutilin resistance in coagulase-negative staphylococci of porcine origin that do not carry known pleuromutilin resistance genes and to determine the localization and genetic environment of the identified resistance gene. Methods: Plasmid DNA of two pleuromutilin-resistant Staphylococcus cohnii and Staphylococcus simulans isolates was transformed into Staphylococcus aureus RN4220. The identified resistance plasmids were sequenced completely. The candidate gene for pleuromutilin resistance was cloned into shuttle vector pAM401. S. aureus RN4220 transformants carrying this recombinant shuttle vector were tested for their MICs. Results: S. cohnii isolate SA-7 and S. simulans isolate SSI1 carried the same plasmid of 5584 bp, designated pSA-7. A variant of the vga(E) gene was detected, which encodes a 524 amino acid ATP-binding cassette protein. The variant gene shared 85.7% nucleotide sequence identity and the variant protein 85.3% amino acid sequence identity with the original vga(E) gene and Vga(E) protein, respectively. The Vga(E) variant conferred cross-resistance to pleuromutilins, lincosamides and streptogramin A antibiotics. Plasmid pSA-7 showed an organization similar to that of the apmA-carrying plasmid pKKS49 from methicillin-resistant S. aureus and the dfrK-carrying plasmid pKKS966 from Staphylococcus hyicus. Sequence comparisons suggested that recombination events may have played a role in the acquisition of this vga(E) variant. Conclusions: A novel vga(E) gene variant was identified, which was located on a small plasmid and was not associated with the transposon Tn6133 [in contrast to the original vga(E) gene]. The plasmid location may enable its further dissemination to other staphylococci and possibly also to other bacteria.

分类号： R45

  • 相关文献

[1]Detection and new genetic environment of the pleuromutilinlincosamidestreptogramin A resistance gene lsa(E) in methicillin-resistant Staphylococcus aureus of swine origin. Li, Beibei,Yao, Jiannan,Shi, Zixue,Wei, Jianchao,Shao, Donghua,Wang, Shaohui,Ma, Zhiyong,Wendlandt, Sarah,Schwarz, Stefan,Liu, Yiqiu,Zhang, Qing. 2013

[2]Midgut transcriptome response to a Cry toxin in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Lei, Yanyuan,Zhu, Xun,Xie, Wen,Wu, Qingjun,Wang, Shaoli,Guo, Zhaojiang,Xu, Baoyun,Zhang, Youjun,Li, Xianchun,Zhou, Xuguo.

[3]Development of a replicative plasmid for gene expression in Mycoplasma bovis. Li, Jiahe,Zhang, Ning,Zhang, Yuewei,Wu, Wenxue,Zhang, Jixiang,Li, Jinxiang.

[4]Characterization of oqxAB in Escherichia coli Isolates from Animals, Retail Meat, and Human Patients in Guangzhou, China. Wang, Jing,Zhi, Chan-Ping,Chen, Xiao-Jie,Guo, Ze-Wen,Liu, Wu-Ling,Luo, Juan,Huang, Xin-Yi,Zeng, Li,Huang, Jia-Wei,Xia, Ying-Bi,Yi, Meng-Ying,Huang, Teng,Zeng, Zhen-Ling,Liu, Jian-Hua,Chen, Xiao-Jie,Liu, Wu-Ling,Zeng, Li. 2017

[5]Construction of pTM series plasmids for gene expression in Brucella species. Tian, Mingxing,Qu, Jing,Bao, Yanqing,Gao, Jianpeng,Liu, Jiameng,Wang, Shaohui,Sun, Yingjie,Ding, Chan,Yu, Shengqing.

[6]Identification of the novel spectinomycin resistance gene spw in methicillin-resistant and methicillin-susceptible Staphylococcus aureus of human and animal origin. Wendlandt, Sarah,Li, Beibei,Lozano, Carmen,Ma, Zhiyong,Torres, Carmen,Schwarz, Stefan. 2013

[7]Plasmid-borne cadmium resistant determinants are associated with the susceptibility of Listeria monocytogenes to bacteriophage. Zhang, Hui,Zhou, Yan,Bao, Hongduo,Zhang, Lili,Wang, Ran,Zhang, Hui,Zhou, Xiaohui.

[8]Complete genomic sequence analyses of Turnip mosaic virus basal-BR isolates from China. Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui,Chen, Jia,Shao, Yun-Hua,Li, Xiang-Dong,Wang, Hong-Yan,Liu, Jin-Liang,Gao, Rui. 2009

[9]Young but not relatively old retrotransposons are preferentially located in gene-rich euchromatic regions in tomato (Solanum lycopersicum) plants. Yingxiu Xu,Jianchang Du.

[10]Evolution of MHC class I genes in two ancient fish, paddlefish (Polyodon spathula) and Chinese sturgeon (Acipenser sinensis). Wang, Dengqiang,Zhong, Lei,Gan, Xiaoni,He, Shunping,Wang, Dengqiang,Wei, Qiwei,Wang, Dengqiang. 2010

[11]GISH characterization of Erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids. Piperidis, Nathalie,Piperidis, George,Chen, Jian-wen,Deng, Hai-hua,Wang, Li-Ping,Jackson, Phillip. 2010

[12]Genomic analysis of a recombinant NADC30-like porcine reproductive and respiratory syndrome virus in china. Wang, Lin-jian,Guo, Zhenhua,Qiao, Songlin,Li, Rui,Xie, Sha,Chen, Xin-xin,Zhang, Gaiping,Wang, Lin-jian,Zhang, Gaiping,Wan, Bo,Zhang, Gaiping. 2018

[13]Genomic organization and sequence dynamics of the AvrPiz-t locus in Magnaporthe oryzae. Li, Ping,Bai, Bin,Zhang, Hong-yan,Zhou, Heng,Zhou, Bo,Li, Ping,Zhou, Heng. 2012

[14]Establishment of a markerless multiple-gene deletion method based on Cre/loxP mutant system for Bacillus pumilus. Guan, Zheng-Bing,Wang, Kai-Qiang,Liao, Xiang-Ru,Shui, Yan. 2017

[15]F gene recombination between genotype II and VII Newcastle disease virus. Sun, Lei,Zhu, Yiping,Kitamura, Yoshihiro,Liu, Wenjun,Qin, Zhuoming,Ma, Baochen,Cui, Zhizhong. 2008

[16]Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus. Xie, Y,Zhou, XP,Zhang, ZK,Qi, YJ. 2002

[17]The impact of positive selection and mutation in prevalence of representative O/Mya-98 foot and mouth disease strains during 2009-2010. Kuang, Wendong,Bai, Xingwen,Qi, Guocai,Hao, Xiaofang,Li, Pinghua,Lu, Zengjun,Bao, Huifang,Sun, Pu,Wu, Lei,Liu, Zaixin. 2012

[18]Identification of a recombinant Muscovy Duck parvovirus (MDPV) in Shanghai, China. Zhu, Yumin,Zhou, Zongqing,Huang, Yu,Yu, Ruisong,Dong, Shijuan,Li, Zhen,Zhu, Yumin,Zhou, Zongqing,Huang, Yu,Yu, Ruisong,Dong, Shijuan,Li, Zhen,Zhang, Yuanshu.

[19]Molecular Characterization of Southern Rice Blacked-Streaked Dwarf Virus (SRBSDV) from Vietnam. Xue, Jin,Li, Jing,Zhang, Heng-Mu,Yang, Jian,Lv, Ming-Fang,Chen, Jian-Ping,Xue, Jin,Gao, Bi-Da,Lv, Ming-Fang. 2014

[20]Genomic analyses reveal that partial sequence of an earlier pseudorabies virus in China is originated from a Bartha-vaccine-like strain. Ye, Chao,Guo, Jin-Chao,Gao, Jia-Cong,Wang, Tong-Yun,Zhao, Kuan,Chang, Xiao-Bo,Wang, Qian,Peng, Jin-Mei,Tian, Zhi-Jun,Cai, Xue-Hui,An, Tong-Qing,Ye, Chao,Tong, Guang-Zhi.

作者其他论文 更多>>

微信小程序