Proteomic Analysis of Schistosoma japonicum Schistosomulum Proteins that are Differentially Expressed Among Hosts Differing in Their Susceptibility to the Infection

文献类型： 外文期刊

第一作者： Hong, Yang

作者： Hong, Yang;Peng, Jinbiao;Jiang, Weibin;Fu, Zhiqiang;Liu, Jinming;Shi, Yaojun;Lin, Jiaojiao;Hong, Yang;Li, Xiangrui

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期刊名称：MOLECULAR & CELLULAR PROTEOMICS （ 影响因子：5.911； 五年影响因子：5.998 ）

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收录情况： SCI

摘要： Schistosomiasis is a tropical, parasitic disease affecting humans and several animal species. The aim of this study was to identify proteins involved in the growth and survival of the parasitic forms inside a host. Schistosomula of Schistosoma japonicum were isolated from three different hosts: the susceptible BALB/c mice; the Wistar rats, which have a considerably lower susceptibility; and the resistant reed vole, Microtus fortis. Soluble proteins of the schistosomula collected from the above three hosts 10 days postinfection were subjected to two-dimensional difference gel electrophoresis. Comparative proteomic analyses revealed that 39, 21, and 25 protein spots were significantly differentially expressed between schistosomula from mice and rats, mice and reed voles, or rats and reed voles, respectively (ANCOVA, p < 0.05). Further, the protein spots were identified by liquid chromatography-tandem MS. Bioinformatics analysis showed that the differentially expressed proteins were essentially those involved in the metabolism of proteins, ribonucleotides, or carbohydrates, or in stress response or cellular movement. This study represents the first attempt at profiling S. japonicum living in different states and provides a basis for a better understanding of the molecular mechanisms in the development and survival of S. japonicum in different host environments.

分类号： Q5

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