Enhanced porcine circovirus Cap protein production by Pichia pastoris with a fuzzy logic DO control based methanol/sorbitol co-feeding induction strategy

文献类型： 外文期刊

第一作者： Zhang, Chunling

作者： Zhang, Chunling;Li, Chunhua;Ni, Jianping;Li, Zhen;Ding, Jian;Gao, Minjie;Hou, Guoli;Liang, Kexue;Shi, Zhongping

作者机构：

关键词： Cap protein;DO;Fermentation;Fuzzy control;Metabolic analysis

期刊名称：JOURNAL OF BIOTECHNOLOGY （ 影响因子：3.307； 五年影响因子：3.778 ）

ISSN：

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收录情况： SCI

摘要： Porcine circovirus Cap protein production by P. pastoris with strong AOX promoter suffered with the problems with traditional pure methanol induction: (1) inefficient methanol metabolism; (2) extensive oxygen supply load; (3) difficulty in stable DO control; (4) low protein titer. In this study, based on the difference of DO change patterns in response to methanol and sorbitol additions, a novel fuzzy control system was proposed to automatically regulate the co-feeding rates of methanol and sorbitol for efficient Cap protein induction. With aid of the proposed control system when setting DO control level at 10%, overall fermentation performance was significantly improved: (1) DO could be stably controlled under mild aeration condition; (2) methanol consumption rate could be restricted at moderate level and the major enzymes involved with methanol metabolism were largely activated; (3) Cap protein concentration reached a highest level of 198 mg/L, which was about 64% increase over the best one using the pure methanol induction strategies. (C) 2014 Elsevier B.V. All rights reserved.

分类号： Q81

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