A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle

文献类型： 外文期刊

第一作者： Liu, Junlong

作者： Liu, Junlong;Guan, Guiquan;Liu, Aihong;Li, Youquan;Yin, Hong;Luo, Jianxun

作者机构：

关键词： Babesia bigemina;Babesia bovis;Multiplex PCR;ITS;ribosomal DNA

期刊名称：ACTA PARASITOLOGICA （ 影响因子：1.44； 五年影响因子：1.335 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19/260) and 5.8% (15/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6/260) and 1.5% (4/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.

分类号： R38

  • 相关文献

[1]Loop-mediated isothermal amplification (LAMP) method based on two species-specific primer sets for the rapid identification of Chinese Babesia bovis and B. bigemina. Liu, Aihong,Guan, Guiquan,Du, Pengfei,Gou, Huitian,Liu, Zhijie,Liu, Junlong,Ma, Milin,Yang, Jifei,Li, Youquan,Niu, Qinli,Ren, Qiaoyun,Bai, Qi,Yin, Hong,Luo, Jianxun.

[2]Fusarium wilt of cauliflower caused by Fusarium equiseti in China. Li, Pan-Liang,Shi, Yan-Xia,Guo, Meng-Yan,Xie, Xue-Wen,Chai, A-Li,Li, Bao-Ju.

[3]Evaluating the Babesia bovis infection of cattle in China with enzyme-linked immunosorbent assay (ELISA). Yang, Congshan,Liu, Junlong,Li, Anyan,Li, Youquan,Liu, Aihong,Xie, Junren,Liu, Guangyuan,Yin, Hong,Guan, Guiquan,Luo, Jianxun,Yin, Hong,Luo, Jianxun.

[4]First report of Babesia bigemina infection in white yaks in China. Qin, Si-Yuan,Wang, Jin-Lei,Ning, Hong-Rui,Tan, Qi-Dong,Yin, Ming-Yang,Zhang, Xiao-Xuan,Zhou, Dong-Hui,Zhu, Xing-Quan,Qin, Si-Yuan,Zhang, Xiao-Xuan,Zhu, Xing-Quan,Ning, Hong-Rui,Tan, Qi-Dong,Yin, Ming-Yang. 2015

[5]Experiments on the transmission of Babesia major and Babesia bigemina by Haemaphysalis punctata. Yin, H,Lu, WS,Luo, JX,Zhang, QC,Lu, WX,Dou, HF. 1996

[6]Discovery of multiple IGS haplotypes within genotypes of Puccinia striiformis. Wang, Yanchun,Hao, Baojun,Zhang, Qiang,Tuo, Enlai,Sun, Guangyu,Zhang, Rong,Zhu, Mingqi,Wang, Yang,Wang, Yanchun,Hao, Baojun,Zhang, Qiang,Tuo, Enlai,Sun, Guangyu,Zhang, Rong,Zhu, Mingqi,Wang, Yang,Wang, Yanchun,Jin, Shelin,Hsiang, Tom. 2012

[7]Long-Term Rice and Green Manure Rotation Alters the Endophytic Bacterial Communities of the Rice Root. Zhang, Xiao-Xia,Gao, Ju-Sheng,Cao, Yan-Hua,Ma, Xiao-Tong,He, Ji-Zheng,Gao, Ju-Sheng.

[8]A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR. Peng, DL,Moens, M.

[9]Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification. Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo,Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo. 2012

[10]Karyotyping of Brassica oleracea L. based on cot-1 and ribosomal DNAs. Wei, Wen-Hui,Zhang, Su-Feng,Wang, Li-Jun,Chen, Bo,Wu, Xiao-Ming,Song, Yun-Chun. 2007

[11]Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Yang, LT,Pan, AH,Zhang, KW,Yin, CS,Qian, BJ,Chen, JX,Huang, C,Zhang, DB. 2005

[12]Development of an allele-specific marker for Glu-B3 alleles in common wheat and establishment of a multiplex PCR assay. Sui, Xinxia,Wang, Linhai,Xia, Xianchun,He, Zhonghu,Sui, Xinxia,Wang, Zhenlin,He, Zhonghu. 2010

[13]Response surface methodology to design a selective co-enrichment broth of Escherichia coli, Salmonella spp. and Staphylococcus aureus for simultaneous detection by multiplex PCR. Zhang, Qiao Yan,Zhou, Yu,Wang, Xiao Fu,Xu, Jun Feng,Zhou, Wen Wu. 2012

[14]Establishment of a Multiplex PCR and an Investigation of Co-Infection Rate of WSSV and IHHNV in Penaeid vannamei in Northern of Jiangsu. Chen, Yi-Bing,Gao, Song,Zhou, Jun-Fang,Wan, Xi-He. 2012

[15]Identification and Quantification of Three Genetically Modified Insect Resistant Cotton Lines Using Conventional and TaqMan Real-Time Polymerase Chain Reaction Methods. Yang, LT,Pan, AH,Zhang, KW,Guo, JC,Yin, CS,Chen, JX,Huang, C,Zhang, DB.

[16]Rapid diagnosis of goose viral infections by multiplex PCR. Chen, Zongyan,Li, Chuanfeng,Li, Guoxin,Yu, Hai,Jiang, Yifeng,Yan, Liping,Meng, Chunchun,Zhou, Yanjun,Tong, Guangzhi,Liu, Guangqing.

[17]Development of a multiplex PCR assay for detection and discrimination of Theileria annulata and Theileria sergenti in cattle. Junlong, Liu,Li, Youquan,Liu, Aihong,Guan, Guiquan,Xie, Junren,Yin, Hong,Luo, Jianxun,Junlong, Liu,Li, Youquan,Liu, Aihong,Guan, Guiquan,Xie, Junren,Yin, Hong,Luo, Jianxun.

[18]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[19]A multiplex reverse transcription-PCR assay for the detection of influenza A virus and differentiation of the H1, H3, H5 and H9 subtypes. Wu, Ling,Ding, Longfei,Pei, Zenglin,Pan, Zishu,Huo, Xixiang,Wen, Guoyuan.

[20]Development and characterization of four moderate multiplex microsatellite panels in crucian carp (Carassius auratus). Cheng, Lei,Lu, Cui-Yun,Li, Chao,Sun, Xiao-Wen,Zhang, Yan. 2013

作者其他论文 更多>>
  • Epidemiology, molecular diversity and zoonotic implications of Echinococcus granulosus sensu stricto (G1) in cattle across Nigeria: Evidence of broader transmission dynamics

    作者：Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Amuda, Tharheer Oluwashola;Li, Li;Wang, Li-Qun;Dai, Guo-Dong;Chen, Wei-Gang;Wu, Yao-Dong;Wu, Ke-Ke;Wu, Yixuan;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong;Yan, Hong-Bin;Morenikeji, Olajumoke Abimbola;Odaibo, Georgina Nijideka;Onoja, Bernard Anyebe;Akanbi, Ibikunle Mark;Yin, Hong;Jia, Wan-Zhong;Fu, Bao-Quan;Luo, Xue-Nong

    关键词：Echinococcus granulosus sensu lato; Cystic echinococcosis; Nigeria; Genetic diversity; Molecular epidemiology; One health

  • A new intermediate host of Echinococcus shiquicus in Qinghai-Tibet Plateau, China

    作者：Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Hou, Xue-Yi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Guo, Min-Kuan;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Li, Wen-Dong;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Chen, Wei-Gang;Li, Li;Dai, Guo-Dong;Wu, Yao-Dong;Amuda, Tharheer Oluwashola;Tian, Xue-Qi;Zhang, Nian-Zhang;Tian, Wen-Jun;Zhang, Lin-Sheng;Li, Le;Fu, Bao-Quan;Yin, Hong;Yan, Hong-Bin;Jia, Wan-Zhong;Shumuye, Nigus Abebe;Gao, Sheng-Zhi;Hou, Xue-Yi;Zhonggecao, Tao;Zhaxilamao, Bao-Quan;Gazangzhuoma, Hong;Doukao, Hong-Bin;Zhang, Fu-Heng;Guo, Min-Kuan;Li, Wen-Dong;Yang, Tao;Fu, Bao-Quan;Yin, Hong;Jia, Wan-Zhong

    关键词：Echinococcus shiquicus; Plateau zokor; Intermediate host; Qinghai-Tibet Plateau; Genetic diversity

  • Expanding the molecular understanding of Theileria infections in cattle and yaks from Gansu and Qinghai, China

    作者：Chai, Yijun;Che, Jin;Zhao, Shuaiyang;Wang, Jinming;Guan, Guiquan;Yin, Hong;Che, Jin;Yin, Hong

    关键词：Molecular prevalence; Theileriosis; Piroplasm; Bovine

  • Lacticaseibacillus rhamnosus G7 alleviates DSS-induced ulcerative colitis by regulating the intestinal microbiota

    作者：Lao, Jianlong;Chen, Man;Yan, Shuping;Gong, Han;Wen, Zhaohai;Yong, Yanhong;Jia, Dan;Lv, Shuting;Zou, Wenli;Li, Junmei;Tan, Huiming;Liu, Zengyuan;Guo, Fucheng;Ju, Xianghong;Li, Youquan;Lao, Jianlong;Ju, Xianghong;Li, Youquan;Jia, Dan;Yin, Hong;Kong, Xiangying

    关键词：Probiotics; Inflammatory; Lacticaseibacillus rhamnosus G7; Gut microbiota

  • First determination of diagnostic doses of the pesticide-sensitive strain of Haemaphysalis longicornis in China

    作者：Diao, Peiwen;Luo, Jin;Ren, Qiaoyun;Wang, Jinming;Cao, Runlai;Guo, Junhui;Guan, Guiquan;Luo, Jianxun;Yin, Hong;Liu, Guangliang;Liu, Guangyuan;Ren, Qiaoyun;Zhang, Yan;Luo, Jianxun;Yin, Hong

    关键词：ticks; pesticide-sensitive strain; Haemaphysalis longicornis; discriminating concentration

  • The African swine fever virus gene MGF_360-4L inhibits interferon signaling by recruiting mitochondrial selective autophagy receptor SQSTM1 degrading MDA5 antagonizing innate immune responses

    作者：Sun, Hualin;Yang, Jifei;Zhang, Zhonghui;Wu, Mengli;Tian, Zhancheng;Liu, Ying;Zhang, Xiaoqiang;Zhong, Jianhao;Yang, Songlin;Chen, Yikang;Luo, Jianxun;Guan, Guiquan;Yin, Hong;Niu, Qingli;Sun, Hualin;Yang, Jifei;Zhang, Zhonghui;Wu, Mengli;Tian, Zhancheng;Liu, Ying;Zhang, Xiaoqiang;Zhong, Jianhao;Yang, Songlin;Chen, Yikang;Luo, Jianxun;Guan, Guiquan;Yin, Hong;Niu, Qingli;Yin, Hong

    关键词：ASFV; MGF_360-4L; SQSTM1; MDA5; mitochondrial autophagy

  • Comparative microRNAs profile of Schistosoma japonicum male worms derived from single-sex and bisexual infections: Implications of the multifunctional role of microRNA

    作者：Zhong, Haoran;Zhu, Danlin;Dong, Bowen;Wu, Luobin;Lu, Ke;Fu, Zhiqiang;Liu, Jinming;Jin, Yamei;Wu, Luobin;Guan, Guiquan;Guan, Guiquan

    关键词：Schistosoma japonicum; MiRNA; Single-sex male worms; Mated male worms

微信小程序