Immobilisation of acid pectinase on graphene oxide nanosheets
文献类型: 外文期刊
第一作者: Liu, Yang
作者: Liu, Yang;Li, Qiang;Feng, Yuan-Yuan;Ji, Geng-Sheng;Li, Tian-Cheng;Tu, Jie;Gu, Xu-Ding;Li, Qiang
作者机构:
关键词: graphene oxide;acid pectinase;enzyme immobilisation;dispersion;loading density
期刊名称:CHEMICAL PAPERS ( 影响因子:2.097; 五年影响因子:1.831 )
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年卷期:
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收录情况: SCI
摘要: Recent progress in nanotechnology has prompted research interest in immobilised enzymes on graphene oxide (GO) nanosheets for their large specific surface area and abundant functional groups. In the present work, acid pectinase was immobilised on the GO via the cross-linking of amino groups on pectinase and functional groups (e.g. carboxyl group) on the GO surface. Acid pectinase was effectively immobilised on the support and high loading densities were obtained (2400 mg per g of support). In addition, the immobilised enzyme achieved a better catalytic efficiency (Kcat/Km) than its free counterpart; 3.7 mg~(?1) min~(?1) mL for immobilised pectinase, 3.5 mg~(?1) min~(?1) mL for free pectinase. Under acidic conditions, pectinase immobilised on GO will be agglomerated, but the addition of surfactant PEG 6000 could solve the problem and afford higher catalytic activity and catalytic efficiency.
分类号: O6
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