cDNA-AFLP analysis reveals heat shock proteins play important roles in mediating cold, heat, and drought tolerance in Ammopiptanthus mongolicus

文献类型： 外文期刊

第一作者： Guo, Huiming

作者： Guo, Huiming;Li, Zhaochun;Zhou, Meiliang;Cheng, Hongmei

作者机构：

关键词： Abiotic stress;cDNA-AFLP;Heat shock protein;RT-PCR;Transcript-derived fragments

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Ammopiptanthus mongolicus (Maxim.ex kom.) Cheng F. is the only evergreen broadleaf shrub endemic to the desert of central Asian and it can survive at drought, salt, and alkali stress. It is believed that A. mongolicus is an important germplasm containing abiotic-tolerance genes. In order to identify drought-, cold-, and heat-responsive genes and to gain a better understanding of stress responses in A. mongolicus, genome-wide investigation of drought-, cold-, and heat-responsive genes was performed in A. mongolicus using cDNA-amplified fragment length polymorphism. Selective amplification with 240 primer combinations generated 5,000 differentially expressed transcript derived fragments (TDFs). Of these, 201 TDFs with differential expression patterns were excised from gels, reamplified by PCR, and sequenced. The gene expression patterns of 11 regulated genes were further investigated by semiquantitative reverse transcriptase polymerase chain reaction analysis. Sequencing and similarity analysis revealed that TDFs present homologies chiefly with proteins involved in various abiotic and biotic stress and developmental responses. The information presented in this study reveals that heat shock proteins play an active role in mediating drought, cold, and heat tolerance in A. mongolicus.

分类号： Q78

  • 相关文献

[1]Comparative expression analysis in susceptible and resistant Gossypium hirsutum responding to Verticillium dahliae infection by cDNA-AFLP. Wang, Sheng-Zheng,Liu, Kai,Si, Ning,Qi, Fang-Jun,Jian, Gui-Liang.

[2]SSAP analysis reveals candidate genes associated with deastringency in persimmon (Diospyros kaki Thunb.) treated with 40 degrees C water. Guan, Changfei,Chen, Li,Chen, Wenxing,Mo, Rongli,Zhang, Qinglin,Liu, Jihong,Luo, Zhengrong,Du, Xiaoyun. 2015

[3]Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP. Xiaoding Ma,Chaozhu Xing,Liping Guo,Yangcang Gong,Hailin Wang,Yunlei Zhao,Jianyong Wu. 2007

[4]The Differential Expression of Resistant Genes Related to Cucumber Scab Displayed by cDNA-AFLP. Sun Xun,Li Xixiang,Qiu Yang,Shen Di,Song Jiangping,Sun Xun,Ren Ruixing. 2010

[5]Tomato defense to the powdery mildew fungus: differences in expression of genes in susceptible, monogenic- and polygenic resistance responses are mainly in timing. Li, Chengwei,Bai, Yuling,Jacobsen, Evert,Visser, Richard,Lindhout, Pim,Bonnema, Guusje. 2006

[6]A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans. Guo, J,Jiang, RHY,Kamphuis, LG,Govers, F. 2006

[7]Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis. Guo Xiao-jun,Rao Qiong,Zhang Fan,Luo Chen,Guo Xiao-jun,Gao Xi-wu,Rao Qiong,Zhang Hong-yu. 2012

[8]Expression Analysis of Low Temperature Responsive Genes in Eupatorium Adenophorum Spreng Using cDNA-AFLP. Wang, Junying,Zhang, Haiwen,Huang, Rongfeng.

[9]Variations of DNA methylation and genes expression for brown fiber during its development process. Li, Zhenpeng,Li, Tingchun,Fan, Honghong,Guo, Ning,Sun, Xu,Cai, Yongping,Lin, Yi,Li, Zhenpeng,Li, Tingchun.

[10]MOLECULAR ANALYSIS OF DIFFERENTIALLY EXPRESSED GENES IN BIRCH (BETULA PLATYPHYLLA) INFLORESCENCE. Sun, Sheng,Kang, Xiu-Ping,Zheng, Shao-Wen,Hao, Rui-Jie,Liu, Qun-Long,Zhang, Jian-Cheng,Xing, Guo-Ming,Tian, Yong-Sheng. 2012

[11]Identification of photoperiod-regulated gene in soybean and functional analysis in Nicotiana benthamiana. Sha Ai-Hua,Shan Zhi-Hui,Zhang Xiao-Juan,Wu Xue-Jun,Qiu De-Zheng,Zhou Xin-An,Chen Yin-Hua,Sha Ai-Hua,Shan Zhi-Hui,Zhang Xiao-Juan,Wu Xue-Jun,Qiu De-Zheng,Zhou Xin-An.

[12]Isolation and identification of a gene in response to rice blast disease in rice. Zheng, XW,Chen, XW,Zhang, XH,Lin, ZZ,Shang, JJ,Xu, JC,Zhai, WX,Zhu, LH.

[13]Identification of candidate genes associated with male sterility in CMS7311 of heading Chinese cabbage (Brassica campestris L. ssp pekinensis). Xu, Xiaoyong,Sun, Xilu,Zhang, Jing,Huang, Weiwei,Zhang, Lugang,Xu, Xiaoyong,Sun, Xilu,Zhang, Jing,Zhang, Lugang,Fang, Zhiyuan,Huang, Weiwei,Fang, Zhiyuan. 2013

[14]Histological and transcriptional characterization of a novel recessive genic male sterility mutant in sesame (Sesamum indicum L.). Wu, Kun,Liu, Hongyan,Zuo, Yang,Yang, Minmin,Zhao, Yingzhong.

[15]Long-Term Boron-Excess-Induced Alterations of Gene Profiles in Roots of Two Citrus Species Differing in Boron-Tolerance Revealed by cDNA-AFLP. Guo, Peng,Yang, Lin-Tong,Ye, Xin,Huang, Jing-Hao,Chen, Li-Song,Guo, Peng,Yang, Lin-Tong,Ye, Xin,Chen, Li-Song,Qi, Yi-Ping,Huang, Jing-Hao,Chen, Li-Song. 2016

[16]cDNA-AFLP analysis reveals the adaptive responses of citrus to long-term boron-toxicity. Guo, Peng,Yang, Lin-Tong,Ye, Xin,Chen, Li-Song,Guo, Peng,Yang, Lin-Tong,Jiang, Huan-Xin,Huang, Jing-Hao,Chen, Li-Song,Qi, Yi-Ping,Jiang, Huan-Xin,Huang, Jing-Hao,Huang, Jing-Hao,Chen, Li-Song,Chen, Li-Song. 2014

[17]Identification of differentially expressed genes related to coloration in red/green mutant pear (Pyrus communis L.). Wu, Jun,Zhao, Guang,Yang, Ya-Nan,Zhang, Shao-Ling,Gu, Chao,Huang, Wen-Jiang,Le, Wen-Quan,Khan, Muhammad Awais. 2013

[18]A Silver-Staining cDNA-AFLP Protocol Suitable for Transcript Profiling in the Latex of Hevea brasiliensis (Para Rubber Tree). Xiao, Xiaohu,Li, Heping,Tang, Chaorong,Xiao, Xiaohu,Li, Heping.

[19]Characterization and binding analysis of a microneme adhesive repeat domain-containing protein from Toxoplasma gondii. Gong, Haiyan,Kobayashi, Kyousuke,Sugi, Tatsuki,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Horimoto, Taisuke,Akashi, Hiroomi,Kato, Kentaro,Gong, Haiyan,Sugi, Tatsuki,Takemae, Hitoshi,Ishiwa, Akiko,Recuenco, Frances C.,Murakoshi, Fumi,Xuan, Xuenan,Kato, Kentaro. 2014

[20]Interaction of heat shock protein 90 B1 (Hsp90B1) with liposome reveals its potential role in protection the integrity of lipid membranes. Li, Pengpeng,Zhang, Muhan,Zou, Ye,Sun, Zhilan,Sun, Chong,Geng, Zhiming,Xu, Weimin,Wang, Daoying. 2018

作者其他论文 更多>>

微信小程序