Core promoter regulates the expression of cathepsin B gene in the fat body of Bombyx mori

文献类型： 外文期刊

第一作者： Cai, Xiang-Yun

作者： Cai, Xiang-Yun;Yu, Jie;Yu, Hai-Yan;Liu, Yan-Wei;Fang, Yin;Ren, Zi-Xu;Jia, Jun-Qiang;Zhang, Guo-Zheng;Guo, Xi-Jie;Gui, Zhong-Zheng;Jia, Jun-Qiang;Zhang, Guo-Zheng;Guo, Xi-Jie;Gui, Zhong-Zheng;Jin, Byung-Rae

作者机构：

关键词： Bombyx mori;Cathepsin B promoter;Dual-luciferase expression system;Regulatory elements

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Bombyx mori cathepsin B (BmCatB) is involved in the programmed cell death of the fat body during B. mori metamorphosis. For a better understanding of the functional regulatory mechanism, the promoter region of BmCatB in the transcriptional regulation has been identified and analyzed in the present study. BmCatB promoter region performed by the 5' truncation or mutagenesis of EcREs was inserted in the pFA3Luc-A3RL double fluorescence expression vector to activate the fireflies luciferase (FLuc) gene. The results indicated that the dual-luciferase activity of BmCatB gene in the silkworm larval fat body is regulated by the length of promoter. Site-directed mutagenesis of EcRE experiment has shown that the EcREs are up-regulated significantly in the regulation of the BmCatB promoter. A 142. bp region (-1165 to -1023) and EcREs are the mainly fat-body tissue-specificity related region and could function as a core promoter element.

分类号： R394

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