Comparison of Lisianthus (Eustoma grandiflorum) Cultivars Based on the Selected Regeneration Media Using Anther Culture

文献类型: 外文期刊

第一作者: Zhou, Xuhong

作者: Zhou, Xuhong;Luo, Ying;Zhou, Xuhong;Mo, Xijun;Qu, Suping;Tian, Min;Wu, Xuewei;Wu, Min;Li, Jinze;Wang, Jihua;Gui, Min

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关键词: calli;genotype;plant regeneration

期刊名称:HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY ( 影响因子:1.842; 五年影响因子:2.083 )

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收录情况: SCI

摘要: Double haploids in lisianthus cultivars were produced using anther culture. The anther of 'Art Peach' was cultured on the Murashige and Skoog (MS) medium with 3% sucrose with auxin and cytokinin. The highest frequency of calli developed from anthers (100%) and green plantlets per100 calli (60 regenerants) was observed on the medium containing 8 mgL~(-1)N6 benzyladenine (BA) and 0.1mg-L~(-1) naphthaleneacetic acids (NAA). When anthers were cultured on the medium supplemented with 2 mg L~(-1) BA and 0.5 mg-L~(-1) NAA, 33.3% of calli formed plantlets. Using the anther culture, callus induction and plantlets regeneration in five cultivars were developed differently, suggesting that the genotype might predetermined anther culture response. These resultsindicate that a highly efficient new microprogation technique can be provided by anther culture in vitro for lisianthus. Anther culture in vitro can provide a highly efficient new microprogation technique for lisianthus.

分类号: S6

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