A MONOCLONAL ANTIBODY-BASED INDIRECT COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETERMINATION OF OLAQUINDOX IN ANIMAL FEED

文献类型： 外文期刊

第一作者： Wang, Lei

作者： Wang, Lei;Zhang, Jingyan;Cui, Dongan;Wang, Xuezhi;Yang, Zhiqiang;Zhang, Kai;Qin, Zhe;Meng, Jiaren;Hao, Guijuan;Li, Jianxi

作者机构：

关键词： Animal feed;Indirect competitive enzyme-linked immunosorbent assay (ELISA);Monoclonal antibody;Olaquindox

期刊名称：ANALYTICAL LETTERS （ 影响因子：2.329； 五年影响因子：1.738 ）

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年卷期：

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收录情况： SCI

摘要： A reliable indirect competitive enzyme-linked immunosorbent assay (ELISA) based on a new specific monoclonal antibody was developed to determine olaquindox in animal feed. The influence of several physicochemical factors (nonfat dried milk solution, organic solvent, incubation time) on the immunoassay was investigated. In the optimized system, the 50% inhibition concentration was 9.66±1.81 μgL~(-1). The limits of detection for porcine, chicken, and fish feed were 0.28, 0.46, and 0.48 μgkg~(-1). The limits of quantification were 1.00 μgkg~(-1) for the feed samples. The recoveries from porcine, chicken, and fish feed spiked with olaquindox were 90–104%, 77–103%, and 78–107%, respectively, with coefficients of variation (CVs) between 3.8 and 14.1%. The cross-reactivity was less than 2.08% with four structurally related compounds and no recognition of five other restricted or forbidden drugs was observed. Parallel analysis of the three spiked feed samples showed comparable results between the indirect competitive ELISA and the standard high-performance liquid chromatography method in China (R~2=0.9985 for porcine feed, R~2=0.9896 for chicken feed, and R~2=0.9987 for fish feed). These data suggest that the developed indirect competitive ELISA is a specific and convenient method and is suitable for olaquindox determination in animal feed.

分类号： O65

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