Molecular cloning and characterization of enhanced disease susceptibility 1 (EDS1) from Gossypium barbadense
文献类型: 外文期刊
第一作者: Su, Xiaofeng
作者: Su, Xiaofeng;Qi, Xiliang;Cheng, Hongmei
作者机构:
关键词: EDS1;Verticillium dahliae;Gossypium barbadense;Verticillium wilt;VIGS
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Arabidopsis enhanced disease susceptibility 1 (EDS1) plays an important role in plant defense against biotrophic and necrotrophic pathogens. The necrotrophic pathogen Verticillium dahliae infection of Gossypium barbadense could lead to Verticillium wilt which seriously reduces the cotton production. Here, we cloned and characterized a G. barbadense homolog of EDS1, designated as GbEDS1. The full-length cDNA of the GbEDS1 gene was obtained by the technique of rapid-amplification of cDNA ends. The open reading frame of the GbEDS1 gene was 1,647 bp long and encoded a protein of 548 amino acids residues. Comparison of the cDNA and genomic DNA sequence of GbEDS1 indicated that this gene contained a single intron and two exons. Like other EDS1s, GbEDS1 contained a conserved N-terminal lipase domain and an EDS1-specific KNEDT motif. Subcellular localization assay revealed that GbEDS1-green fluorescence protein fusion protein was localized in both cytosol and nucleus. Interestingly, the transcript levels of GbEDS1 were dramatically increased in response to pathogen V. dahliae infection. To investigate the role of GbEDS1 in plant resistance against V. dahliae, a conserved fragment derived from GbEDS1 was used to knockdown the endogenous EDS1 in Nicotiana benthamiana by heterologous virus-induced gene silencing. Our data showed that silencing of NbEDS1 resulted in increased susceptibility to V. dahliae infection in N. benthamiana, suggesting a possible involvement of the novelly isolated GbEDS1 in the regulation of plant defense against V. dahliae.
分类号: Q7
- 相关文献
作者其他论文 更多>>
-
Detoxification and neurotransmitter clearance drive the recovery of Arma chinensis from ll-cypermethrin-triggered knockdown
作者:Wang, Zhen;He, Wenjie;Fu, Luyao;Cheng, Hongmei;Lin, Changjin;Liu, Chenxi;Dong, Xiaolin
关键词:ll-cypermethrin; Trans-regulation; Recovery; Detoxification; Neurotransmitter
-
Genome-wide analysis of gustatory receptor genes and identification of the fructose gustatory receptor in Arma chinensis
作者:Wang, Zhen;Liu, Dianyu;Ma, Le;Cheng, Hongmei;Lin, Changjin;Fu, Luyao;Chen, Yu;Liu, Chenxi;Liu, Dianyu;Ma, Le;Chen, Yu;Dong, Xiaolin
关键词:Gustatory receptor; Fructose; Arma chinensis
-
Preparation of salicylic acid nano-protectant with dual synergistic mechanism: High direct fungicidal activity and plant defence toward cotton Verticillium wilt
作者:Yin, Jiaming;Zhao, Jiajia;Xue, Fan;Shen, Jie;Yan, Shuo;Yin, Jiaming;Zhao, Jiajia;Wang, Zeng;Xue, Fan;Shen, Jie;Yan, Shuo;Wang, Qi;Guo, Huiming;Cheng, Hongmei;Su, Xiaofeng;Guo, Huiming;Cheng, Hongmei;Su, Xiaofeng;Li, Jie;Yin, Meizhen
关键词:Drug delivery; Fungicidal activity; Nanocarrier; Nano-protectant; Plant defense
-
AmCBF1 activates the expression of GhClpR1 to mediate dark-green leaves in cotton (Gossypium hirsutum)
作者:Zhang, Qianqian;Wang, Peilin;Li, Weilong;Liu, Man;Zhou, Lili;Su, Xiaofeng;Cheng, Hongmei;Guo, Huiming;Zhang, Qianqian;Liu, Man;Wang, Peilin;Cheng, Hongmei;Guo, Huiming;Zhou, Lili
关键词:Cotton; Chloroplast; GhClpR1; AmCBF1; Transcription factor
-
A 5.2-kb insertion in the coding sequence of PavSCPL, a serine carboxypeptidase-like enhances fruit firmness in Prunus avium
作者:Qi, Xiliang;Dong, Yuanxin;Liu, Congli;Song, Lulu;Chen, Lei;Li, Ming;Liu, Congli
关键词:sweet cherry; fruit firmness; BSA-seq; PavSCPL; molecular marker
-
A Verticillium dahliae exoglucanase as potential HIGS target interacts with a cotton cysteine protease to confer resistance to cotton Verticillium wilt
作者:Su, Xiaofeng;Wang, Qi;Guo, Huiming;Cheng, Hongmei;Zhang, Tao;Ge, Xiaoyang;Liu, Wende;Li, Zhiqiang;Wang, Xingfen;Sun, Zhengwen
关键词:Verticillium wilt; Verticillium dahliae; VdEXG; host-induced gene silencing; GhRD21A
-
Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding
作者:Wang, Peilin;Guo, Huiming;Wang, Peilin;Abbas, Mubashir;Cheng, Hongmei;Guo, Huiming;He, Jianhan;Zhou, Lili
关键词:cotton; sequencing; diversity; mutant library; germplasm resources