Molecular cloning, sequence identification, and gene expression analysis of bovine ADCY2 gene

文献类型： 外文期刊

第一作者： Li, Y. X.

作者： Li, Y. X.;Seo, K. S.;Jin, H. G.;Yan, C. G.;Jin, X.;Ren, C. Y.;Jiang, C. J.;Jin, C. D.

作者机构：

关键词： ADCY2;Gene cloning;Sequence analysis;Gene expression;Cattle

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Adenylyl cyclase 2 (ADCY2), a class B member of adenylyl cyclases, is important in accelerating phosphor-acidification as well as glycogen synthesis and breakdown. Given its distinct role in flesh tenderization after butchering, we cloned and sequenced the ADCY2 gene from Yanbian cattle and assessed its expression in bovine tissues. A 2947 bp nucleotide sequence representing the full-length cDNA of bovine ADCY2 gene was obtained by 5' and 3' remote analysis computations for gene expression. Analyses of the putative protein sequence showed that ADCY2 had high homology among species, except with the non-mammal Oreochromis niloticus. Gene structural domain analyses in humans and rats indicated that the ADCY2 protein had no flaw; only the transmembrane domain was reduced and the CYCc structure domain was shortened. Assessment of ADCY2 expression in bovine tissues by real-time PCR showed that the highest expression was in the testes, followed by the longissimus dorsi, tensor fasciae latae, and latissimus dorsi. These data will serve as a foundation for further insight into the cattle ADCY2 gene.

分类号： Q7

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