Valid application of western blotting

文献类型： 外文期刊

第一作者： Hu, Xiuli

作者： Hu, Xiuli;Chen, Yanhui;Wu, Liuji;Hu, Xiuli;Chen, Yanhui;Wu, Liuji;Chen, Yanhui;Tang, Haitao;Han, Zanping

作者机构：

关键词： Western blotting;Systematic errors;Reference proteins;Normalization;Accuracy

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. However, the systematic errors in the application of western blotting analysis are frequently to be found, which may compromise the interpretation of results. To make a valid application of western blotting, it is essential to begin with three independent biological replicates. Subsequently, a more reliable normalization method is in urgent need for western blotting analysis and using reference proteins is the currently preferred method of normalization. Additionally, identification of valid reference proteins is crucial for western blotting analysis and it should be examined carefully in relation to the cell or tissue types when using housekeeping proteins as internal standards.

分类号： Q7

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