Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene

文献类型： 外文期刊

第一作者： Lian, Ling

作者： Lian, Ling;Wang, Xiaowei;Zhu, Yongsheng;He, Wei;Cai, Qiuhua;Xie, Huaan;Zhang, Jianfu;Lian, Ling;Wang, Xiaowei;Zhu, Yongsheng;He, Wei;Cai, Qiuhua;Xie, Huaan;Zhang, Jianfu;Lian, Ling;Wang, Xiaowei;Zhu, Yongsheng;He, Wei;Cai, Qiuhua;Xie, Huaan;Zhang, Jianfu;Lian, Ling;Wang, Xiaowei;Zhu, Yongsheng;He, Wei;Cai, Qiuhua;Xie, Huaan;Zhang, Jianfu;Zhang, Muqing

作者机构：

关键词： Phosphoenolpyruvate carboxylase gene;(PEPC gene) C4 photosynthesis;Gene expression;Transgenic rice

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Phosphoenolpyruvate carboxylase (PEPC) is known to play a key role in the initial fixation of CO2 in C4 photosynthesis. The PEPC gene from sugarcane (a C4 plant) was introduced into indica rice (Hang2), a process mediated by Agrobacterium tumefaciens. Integration patterns and copy numbers of the gene was confirmed by DNA blot analysis. RT-PCR and western blotting results showed that the PEPC gene was expressed at both the mRNA and protein levels in the transgenic lines. Real-time PCR results indicated that expression of the sugarcane PEPC gene occurred mostly in green tissues and changed under high temperature and drought stress. All transgenic lines showed higher PEPC enzyme activities compared to the untransformed controls, with the highest activity (11.1 times higher than the controls) being observed in the transgenic line, T34. The transgenic lines also exhibited higher photosynthetic rates. The highest photosynthetic rate was observed in the transgenic line, T54 (22.3 mu mol m(-2) s(-1); 24.6 % higher than that in non-transgenic plants) under high-temperature conditions. Furthermore, the filled grain and total grain numbers for transgenic lines were higher than those for non-transgenic plants, but the grain filling (%) and 1,000-grain weights of all transgenic lines remained unchanged. We concluded that over-expression of the PEPC gene from sugarcane in indica rice (Hang2) resulted in higher PEPC enzyme activities and higher photosynthesis rates under high-temperature conditions.

分类号： Q7

  • 相关文献

[1]Morphology and photosynthetic enzyme activity of maize phosphoenolpyruvate carboxylase transgenic rice. Li, W. C.,Wang, J.,Sun, Y. L.,Ji, S. D.,Guo, S. W.. 2015

[2]The mRNA abundance of pepc2 gene is negatively correlated with oil content in Chlamydomonas reinhardtii. Deng, Xiaodong,Li, Yajun,Fei, Xiaowen,Fei, Xiaowen. 2011

[3]Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference. Ma, Jin,Song, Yunzhi,Wu, Bin,Li, Kaidong,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong. 2011

[4]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[5]Dimeric artificial microRNAs mediate high resistance to RSV and RBSDV in transgenic rice plants. Sun, Lin,Lin, Chao,Du, Jinwen,Song, Yunzhi,Liu, Hongmei,Zhou, Shumei,Wen, Fujiang,Zhu, Changxiang,Jiang, Mingsong.

[6]Characteristics of CO2 exchange and chlorophyll fluorescence of transgenic rice with C-4 genes. Huang, XQ,Jiao, DM,Chi, W,Ku, MSB. 2002

[7]The characteristics of CO2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice. Jiao, DM,Li, X,Huang, XQ,Wei, C,Kuang, TY,Maurice, KSB. 2001

[8]Establishment of a rice transgene flow model for predicting maximum distances of gene flow in Southern China. Yao, Kemin,Hu, Ning,Chen, Wanlong,Li, Renzhong,Yuan, Qianhua,Wang, Feng,Qian, Qian. 2008

[9]Expression of an elicitor-encoding gene from Magnaporthe grisea enhances resistance against blast disease in transgenic rice. Qiu, Dewen,Mao, Jianjun,Yang, Xiufen,Zeng, Hongmei. 2009

[10]Isolation of the endosperm-specific LPAAT gene promoter from coconut (Cocos nucifera L.) and its functional analysis in transgenic rice plants. Zheng, Yusheng,Wang, Zhekui,Li, Dongdong,Xu, Li,Zhou, Peng,Ye, Rongjian,Lin, Yongjun,Ye, Rongjian,Lin, Yongjun. 2010

[11]Lethal and Sub-Lethal Effects of Transgenic Rice Containing cry1Ac and CpTI Genes on the Pink Stem Borer, Sesamia inferens (Walker). Han Lan-zhi,Hou Mao-lin,Wu Kong-ming,Peng Yu-fa,Wang Feng. 2011

[12]Event-specific qualitative and quantitative detection of transgenic rice Kefeng-6 by characterization of the transgene flanking sequence. Wang, Wei-Xia,Lai, Feng-Xiang,Fu, Qiang,Zhu, Ting-Heng. 2011

[13]Transgenic fertile japonica rice plants expressing a modified crylA(b) gene resistant to yellow stem borer. Wu, C,Fan, Y,Zhang, C,Oliva, N,Datta, SK. 1997

[14]Impacts of transgenic cry1Ab rice on non-target planthoppers and their main predator Cyrtorhinus lividipennis (Hemiptera : Miridae) - A case study of the compatibility of Bt rice with biological control. Liu, Zhi-Cheng,Ye, Gong-yin,Shen, Zhi-cheng,Hu, Cui,Peng, Yu-fa,Altosaar, Illimar,Shelton, Anthony M.. 2007

[15]Functional and numerical responses of Cyrtorhinus lividipennis to eggs of Nilaparvata lugens are not affected by genetically modified herbicide-tolerant rice. Huang Qian,Long Li-ping,Ling Yan,Huang Suo-sheng,Wu Bi-qiu,Huang Feng-kuan,Cai Jian-he,Chen Yu-chong,Xiao Guo-ying. 2015

[16]Efficient Agrobacterium-mediated transformation of rice by phosphomannose isomerase/mannose selection. Ding Zai-Song,Zhao Ming,Jing Yu-Xiang,Li Liang-Bi,Kuang Ting-Nin. 2006

[17]Characterization of competitive interactions in the coexistence of Bt-transgenic and conventional rice. Liu, Yongbo,Li, Junsheng,Ge, Feng,Liang, Yuyong,Wu, Gang. 2015

[18]Ectopic expression of the spike protein of Rice Ragged Stunt Oryzavirus in transgenic rice plants inhibits transmission of the virus to insects. Shao, CG,Wu, JH,Zhou, GY,Sun, G,Peng, BZ,Lei, JL,Jin, DD,Chen, SX,Upadhyaya, NM,Waterhouse, P,Gong, ZX. 2003

[19]Characterization of OsDREB6 responsive to osmotic and cold stresses in rice. Ke, Ya-Guang,Yang, Zhi-Jun,Luo, Li-Jun,Ke, Ya-Guang,Yu, Shun-Wu,Li, Tian-Fei,Wu, Jin-Hong,Gao, Huan,Luo, Li-Jun,Fu, Ya-Ping.

[20]Baseline susceptibility of Cnaphalocrocis medinalis (Lepidoptera : Pyralidae) to Bacillus thuringiensis toxins in China. Hou, M. L.,Peng, Y. F.,Liu, P. L..

作者其他论文 更多>>

微信小程序