Determination of global DNA methylation level by capillary electrophoresis using octyl-modified quaternized cellulose as an electrolyte additive

文献类型： 外文期刊

第一作者： Huang, Dihui

作者： Huang, Dihui;Yang, Qin;Jin, Shanxia;Zhou, Ping;Deng, Qianchun

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关键词： DNAmethylation;5-Methylcytosine;Octyl-modified quaternized cellulose;CE

期刊名称：ANALYTICAL AND BIOANALYTICAL CHEMISTRY （ 影响因子：4.142； 五年影响因子：3.863 ）

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收录情况： SCI

摘要： A new cellulose derivative, octyl-modified quaternized cellulose (OMQC), was synthesized and used as electrolyte additive for the analysis of 5-methylcytosine by capillary electrophoresis with UV detection. While added in the background electrolyte, OMQC carrying octyl groups and quaternary ammonium groups exhibited dynamic coating ability. Capillary coated with OMQC was able to generate a stable anodal electro-osmotic flow even at pH 12.0. After several running conditions were optimized, a new method for quantification of genomic methylation level was developed on the basis of hydrolysis of DNA by formic acid and separation of nucleic acid bases by capillary electrophoresis. Cytosine and 5-methylcytosine were separated with a resolution near 4.0 in less than 10 min. The detection limits (S/N=3) were 1.1 and 1.5 μg/mL for cytosine and 5-methylcytosine, respectively.

分类号： O65

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