Simultaneous quantitation of nucleosides, nucleobases, amino acids, and alkaloids in mulberry leaf by ultra high performance liquid chromatography with triple quadrupole tandem mass spectrometry

文献类型： 外文期刊

第一作者： Zhang, Li-li

作者： Zhang, Li-li;Shu, Shu-lan;Qian, Da-wei;Duan, Jin-ao;Zhang, Li-li;Shu, Shu-lan;Qian, Da-wei;Duan, Jin-ao;Bai, Yong-liang;Zhen Ou-yang;Liu, Li

作者机构：

关键词： Alkaloids;Amino acids;Morus Alba;Nucleosides;Simultaneous quantitation

期刊名称：JOURNAL OF SEPARATION SCIENCE （ 影响因子：3.645； 五年影响因子：2.943 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： An ultra high performance liquid chromatography coupled with a triple quadrupole mass detection and electrospray ionization mass spectrometry method has been established for the simultaneous determination of the 14 nucleosides and nucleobases, 24 amino acids and two main alkaloids in mulberry leaf. In this method, a complicated mobile phase, the flow rate of which was 0.4 mL/min, was applied to the gradient elution, which provided a satisfied separation of the 40 compounds. The present method was validated, and sufficient reproducibility and accuracy were obtained for the quantitative measurement of the 40 compounds. The method was subsequently applied to ten mulberry leaves and the results showed that almost all of these samples were rich in nucleosides, nucleobases, amino acids, and alkaloids. The proposed method, which is convenient and economical, could serve as a prerequisite for the quality control of mulberry leaf herbs and be applied analogously to other Chinese medicines.

分类号： O6

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