EFFECT OF STRAW LEACHATES FROM CRY1CA-EXPRESSING TRANSGENIC RICE ON THE GROWTH OF CHLORELLA PYRENOIDOSA

文献类型： 外文期刊

第一作者： Zhu, Haojun

作者： Zhu, Haojun;Ding, Jiatong;Wang, Jiamei;Chen, Xiuping;Li, Yunhe;Zhu, Haojun;Peng, Yufa

作者机构：

关键词： Bt-rice straw;Cry1Ca protein;Chlorella pyrenoidosa;Safety assessment;Nontarget effect

期刊名称：ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY （ 影响因子：3.742； 五年影响因子：3.866 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Because of the prevalence of algae in rice paddy fields, they will be exposed to Bacillus thurigiensis (Bt) proteins released from Bt protein-expressing genetically engineered rice. To assess the effects of leachates extracted from Cry1Ca-expressing transgenic rice (T1C-19) straw on the microalga Chlorella pyrenoidosa, the authors added purified Cry1Ca (10 μg/L, 100 μg/L, and 1000 μg/L) and 5 concentrations of diluted extracts (5%, 10%, 20%, 40%, and 80%) from T1C-19 and the nontransformed control strain Minghui 63 (MH63) to the medium of C. pyrenoidosa. The authors found that the growth curves of C. pyrenoidosa treated with purified Cry1Ca overlapped with the medium control; that the order of C. pyrenoidosa growth rates for the T1C-19 leachate concentrations was 5% ＞ 10% ＞ 20% ＞ control ＞ 40% ＞ 80%, and for the MH63 concentrations the order was 5% ＞ 10% ＞ control ＞ 20% ＞ 40% ＞ 80%, but there were no statistical differences between the 20% T1C-19 or 20% MH63 leachate treatment and the medium control on day 8; and that after 7 d of culture, Cry1Ca could be detected in C. pyrenoidosa treated with different concentrations of T1C-19 leachate. The results demonstrated that Cry1Ca protein released from T1C-19 rice can be absorbed into C. pyrenoidosa but that purified Cry1Ca and leachates from T1C-19 rice have no obvious adverse effects on the growth of C. pyrenoidosa.

分类号： R99`X`R12

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