Recombinant production of the antimicrobial peptide NZ17074 in Pichia pastoris using SUMO3 as a fusion partner

文献类型： 外文期刊

第一作者： Wang, X. J.

作者： Wang, X. J.;Wang, X. M.;Teng, D.;Zhang, Y.;Mao, R. Y.;Wang, J. H.;Wang, X. J.;Wang, X. M.;Teng, D.;Zhang, Y.;Mao, R. Y.;Wang, J. H.

作者机构：

关键词： antimicrobial activity;antimicrobial peptide;NZ17074;Pichia pastoris;recombinant expression;SUMO3

期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.858； 五年影响因子：2.776 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The antimicrobial peptide NZ17074, which is derived from arenicin-3 isolated from Arenicola marina, displayed high activity against a broad range of pathogenic bacteria and fungi. However, NZ17074 has not been produced using fermentation technology. The aim of this work was to study the expression of difficult-to-express NZ17074 in Pichia pastoris by fusing with SUMO3. The DNA fragments of NZ17074 and SUMO3 were fused into SUMO3-NZ17074 using overlap PCR and cloned into the pPICZA vector to construct the pPICZ-SUMO3-NZ17074 expression vector. The rSUMO3-NZ17074 fusion protein, purified by Ni2+-chelating affinity chromatography, was cleaved by 50% formic acid at 50 degrees C for 28h to release recombinant NZ17074 (rNZ17074). After purification with second affinity column, 4 center dot 1mg rNZ17074 peptide with the purity over 90% was obtained from per litre fermentation culture. The rNZ17074 peptide exhibited the significant inhibition activity against Gram-negative bacteria: its minimal inhibitory concentrations (MICs) against Escherichia coli, Salmonella enteritidis and Pseudomonas aeruginosa were 2-4, 2 and 8-16gml-1, respectively, which indicated that SUMO3 is a good fusion partner for the expression of the toxic peptide

分类号： Q939

  • 相关文献

[1]Candidacidal mechanism of the arenicin-3-derived peptide NZ17074 from Arenicola marina. Wang, Xiaojie,Wang, Xiumin,Teng, Da,Zhang, Yong,Mao, Ruoyu,Xi, Di,Wang, Jianhua,Wang, Xiaojie,Wang, Xiumin,Teng, Da,Zhang, Yong,Mao, Ruoyu,Xi, Di,Wang, Jianhua.

[2]Deleting the first disulphide bond in an arenicin derivative enhances its expression in Pichia pastoris. Yang, N.,Wang, X.,Teng, D.,Mao, R.,Hao, Y.,Wang, J.,Yang, N.,Wang, X.,Teng, D.,Mao, R.,Hao, Y.,Wang, J.,Feng, X..

[3]Modification and characterization of a new recombinant marine antimicrobial peptide N2. Yang, Na,Feng, Xingjun,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Hao, Ya,Zong, Lifen,Wang, Jianhua,Yang, Na,Wang, Xiumin,Teng, Da,Mao, Ruoyu,Hao, Ya,Zong, Lifen,Wang, Jianhua. 2016

[4]Contribution of bovine lactoferrin inter-lobe region to iron binding stability and antimicrobial activity against Staphylococcus aureus. Bai, Xuejing,Teng, Da,Tian, Zigang,Zhu, Yanping,Yang, Yalin,Wang, Jianhua,Bai, Xuejing,Teng, Da,Tian, Zigang,Zhu, Yanping,Yang, Yalin,Wang, Jianhua.

[5]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[6]The recombinant serine protease XAoz1 of Arthrobotrys oligospora exhibits potent nematicidal activity against Caenorhabditis elegans and Haemonchus contortus. Wang Junwei,Meng Qingling,Qiao Jun,Wang Weisheng,Chen Shuangqing,Zhao Chunguang,Chen Chuangfu,Luo Jianxun.

[7]Purification and characterization of a novel antimicrobial peptide from Brevibacillus laterosporus strain A60. Guo, Lihua,Zeng, Hongmei,Yang, Xiufen,Yuan, Jingjing,Shi, Huaixing,Xiong, Yehui,Chen, Mingjia,Han, Lei,Qiu, Dewen.

[8]Two novel antimicrobial peptides purified from the symbiotic bacteria Xenorhabdus budapestensis NMC-10. Xiao, Yao,Meng, Fanlu,Qiu, Dewen,Yang, Xiufen.

[9]Design and recombination expression of a novel plectasin-derived peptide MP1106 and its properties against Staphylococcus aureus. Cao, Xintao,Zhang, Yong,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Wang, Jianhua,Cao, Xintao,Zhang, Yong,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Wang, Jianhua.

[10]GAP-initiated constitutive expression of a novel plectasin-derived peptide MP1106 by Pichia pastoris and its activity against Streptococcus suis. Jiao, Jian,Feng, Xingjun,Jiao, Jian,Mao, Ruoyu,Wang, Xiumin,Zhang, Yong,Teng, Da,Wang, Jianhua,Jiao, Jian,Mao, Ruoyu,Wang, Xiumin,Zhang, Yong,Teng, Da,Wang, Jianhua. 2015

[11]Expression of plectasin in Pichia pastoris and its characterization as a new antimicrobial peptide against Staphyloccocus and Streptococcus. Wang, Jianhua.

[12]Biotechnical paving of recombinant enterocin A as the candidate of anti-Listeria agent. Hu, Xiaoyuan,Mao, Ruoyu,Zhang, Yong,Teng, Da,Wang, Xiumin,Xi, Di,Wang, Jianhua,Hu, Xiaoyuan,Mao, Ruoyu,Zhang, Yong,Teng, Da,Wang, Xiumin,Xi, Di,Wang, Jianhua,Hu, Xiaoyuan,Huang, Jianzhong. 2014

[13]Expression of Apostichopus japonicus lysozyme in the methylotrophic yeast Pichia pastoris. Wang, Tingting,Xu, Yongping,Liu, Wenjia,Jin, Liji,Xu, Yongping,Jin, Liji,Sun, Yongxin,Wang, Tingting,Xu, Yongping,Liu, Wenjia,Sun, Yongxin,Jin, Liji.

[14]In silico analysis and recombinant expression of BamA protein as a universal vaccine against Escherichia coli in mice. Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua,Guan, Qingfeng,Wang, Xiao,Wang, Xiumin,Teng, Da,Wang, Jianhua.

[15]Production of Bacteriocin E50-52 by Small Ubiquitin-Related Modifier Fusion in Escherichia coli. Wang, Qing,Fu, Wenjuan,Ma, Qingshan,Yu, Zhanqiao,Zhang, Rijun. 2013

[16]Design and high-level expression of a hybrid antimicrobial peptide LF15-CA8 in Escherichia coli. Feng, Xing-Jun,Xing, Li-Wei,Liu, Di,Song, Xue-Ying,Li, Jing,Xu, Wen-Shan,Liu, Chun-Long,Li, Zhong-Qiu. 2014

[17]Molecular characters and recombinant expression of the carboxylesterase gene of the meadow moth Loxostege sticticalis L. (Lepidoptera: Pyralidae). Zhong, Tao,Li, Ke-Bin,Cao, Ya-Zhong,Guo, Wei,Wei, Zhao-Jun. 2011

[18]Recombinant expressions of sweet plant protein mabinlin II in Escherichia coli and food-grade Lactococcus lactis. Gu, Wenliang,Xia, Qiyu,Fu, Shaoping,Guo, Jianchun,Yao, Jing,Hu, Xinwen.

[19]High-Yield Recombinant Expression of the Chicken Antimicrobial Peptide Fowlicidin-2 in Escherichia coli. Feng, Xingjun,Xu, Wenshan,Qu, Pei,Li, Xiaochong,Xing, Liwei,Liu, Di,Jiao, Jian,Wang, Jue,Li, Zhongqiu,Liu, Chunlong,Liu, Chunlong.

[20]Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity. Deng Li-hua,Weng Lu-shui,Deng Xiang-yang,Xiao Guo-ying,Wang Zuo-ping,Fu Xi-qin,Xin Ye-yun. 2017

作者其他论文 更多>>

微信小程序