OsLOX2, a rice type I lipoxygenase, confers opposite effects on seed germination and longevity

文献类型： 外文期刊

第一作者： Huang, Jiexue

作者： Huang, Jiexue;Cai, Maohong;Long, Qizhang;Liu, Linglong;Lin, Qiuyun;Jiang, Ling;Chen, Saihua;Wan, Jianmin;Wan, Jianmin

作者机构：

关键词： Oryza sativa L.;OsLOX2;Seed germination;Seed longevity;Grain storage

期刊名称：TRANSGENIC RESEARCH （ 影响因子：2.788； 五年影响因子：2.377 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Rice production and seed storage are confronted with grain deterioration and loss of seed viability. Some members of the lipoxygenase (LOX) family function in degradation of storage lipids during the seed germination, but little is known about their influence on seed longevity during storage. We characterized the role of rice OsLOX2 gene in seed germination and longevity via over-expression and knock-down approaches. Abundant expression of OsLOX2 was detected in panicles, roots, and stems, but not in leaves. Moreover, OsLOX2 was highly induced during germination. OsLOX2 protein, located in the cytoplasm, showed a wide range of temperature adaptation (20-50 A degrees C) and a substrate preference to linoleic acid. Lines over-expressing OsLOX2 showed accelerated seed germination under normal condition and lower seed viability after accelerated aging. RNA interference (RNAi) of OsLOX2 caused delayed germination and enhanced seed longevity. RNAi lines with strongly repressed OsLOX2 activity completely lost the capability of germination after accelerated aging. More lipid hydroperoxide were found in OE15 than the control, but less in RNAi lines than in the WT Nipponbare. Therefore, OsLOX2 acts in opposite directions during seed germination and longevity during storage. Appropriate repression of the OsLOX2 gene may delay the aging process during the storage without compromising germination under normal conditions.

分类号： R394

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