An inactivated recombinant rabies CVS-11 virus expressing two copies of the glycoprotein elicits a higher level of neutralizing antibodies and provides better protection in mice

文献类型: 外文期刊

第一作者: Xue, Xiang-Hong

作者: Xue, Xiang-Hong;Li, Ling;Yang, Song-Tao;Xia, Xian-Zhu;Xue, Xiang-Hong;Zheng, Xue-Xing;Wang, Hua-Lei;Ma, Jin-Zhu;Li, Ling;Gai, Wei-Wei;Wang, Tie-Cheng;Yang, Song-Tao;Xia, Xian-Zhu;Zheng, Xue-Xing;Ma, Jin-Zhu

作者机构:

关键词: G protein;Inactivated vaccines;Pathogenicity;Rabies virus

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

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年卷期:

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收录情况: SCI

摘要: The rabies virus (RABV) G protein is the primary contributor to the pathogenicity and protective immunity of RABV. In this study, we generated a recombinant rCVS-11-G strain containing two copies of the G protein derived from the pathogenic wild-type (wt) CVS-11 strain and based on its infectious clone. Compared with the wtCVS-11 strain, the rCVS-11-G strain possessed a larger virion and 1.4-fold more G protein, but it exhibited a similar growth property to the rCVS-11 strain, including passaging stability in vitro. qPCR results showed that the two G genes were over-expressed in BHK-21 cells infected with the rCVS-11-G strain. However, the rCVS-11-G strain presented an 80% lower LD50 than the wtCVS-11 strain when intracranially (i.c.) inoculated in adult mice. Adult mice that were either intracranially (i.c.) or intramuscularly (i.m.) inoculated with rCVS-11-G strain developed more acute neurological symptoms and greater mortality than those inoculated with the wtCVS-11 strain. Furthermore, the rCVS-11-G strain was more easily and rapidly taken up by neuroblastoma cells. These data indicated that the rCVS-11-G strain might have increased neurotropism because of the over-expression of the pathogenic G protein. The inactivated rCVS-11-G strain induced significantly higher levels of virus neutralization antibodies and provided better protection from street rabies virus challenge in mice. Therefore, the rCVS-11-G strain may be a promising inactivated vaccine strain due to its better immunogenicity.

分类号: R37

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