Expression of the GPRC5D gene in Liaoning Cashmere goats

文献类型: 外文期刊

第一作者: Jin, Mei

作者: Jin, Mei;Li, XiuNa;Piao, Jun;Qu, YangLe;Wang, YiMeng;Piao, JingAi;Chu, MingXing

作者机构:

关键词: GPRC5D;bioinformatics;RT-PCR;in situ hybridization

期刊名称:TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES ( 影响因子:0.581; 五年影响因子:0.78 )

ISSN:

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收录情况: SCI

摘要: This study aimed to characterize the expression the GPRC5D gene in the skin of the Liaoning Cashmere goat. Initially, the GPRC5D gene sequence vi^as subjected to bioinformatics analysis. Second, semiquantitative RT-PCR was used to detect GPRC5D gene expression in the tissues. Third, in situ hybridization was used to locate GPRC5D gene expression in the primary and secondary hair follicles. Functional site analysis showed that GPRC5D has 7 transmembrane domains. RT-PCR showed that GPRC5D was expressedonly in the skin. In situ hybridization showed that GPRC5D was expressed in the inner root sheath only. We speculated that GPRC5D may regulate cashmere growth.

分类号: S85

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