Genome-wide analysis of Water-stress-responsive microRNA expression profile in tobacco roots

文献类型： 外文期刊

第一作者： Yin, Fuqiang

作者： Yin, Fuqiang;Gao, Jian;Qin, Cheng;Zhang, Zhiming;Shen, Yaou;Lin, Haijian;Pan, Guangtang;Yin, Fuqiang;Liu, Ming;Zhang, Wenyou;Xia, Mingzhong;Qin, Cheng;Yang, Aiguo;Luo, Chenggang

作者机构：

关键词： MicroRNAexpression profile;Tobacco;PEG treatment;Q-PCR

期刊名称：FUNCTIONAL & INTEGRATIVE GENOMICS （ 影响因子：3.41； 五年影响因子：3.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) play a pivotal role in posttranscriptional regulation of gene expression in plants. In this study, we investigate miRNAs in an agronomically important common tobacco in China, named Honghua Dajinyuan (a drought-tolerant cultivar). Here, we report a comprehensive analysis of miRNA expression profiles in mock-treat grown (CK) and 20 % polyethylene glycol-grown (PEG-grown) tobacco roots using a high-throughput sequencing approach. A total of 656 unique miRNAs representing 53 miRNA families were identified in the two libraries, of which 286 unique miRNAs representing 162 microRNAs were differentially expressed. In addition, nine differentially expressed microRNAs selected from different expressed miRNA family with high abundance were subjected to further analysis and validated by quantitative real-time PCR (Q-PCR). In addition, the expression pattern of these identified candidate conserved miRNA and target genes of three identified miRNA (ntamiR172b, nta-miR156i, and nta-miR160a) were also validated by Q-PCR. Gene ontology (GO) enrichment analysis suggests that the putative target genes of these differentially expressed miRNAs are involved in metabolic process and response to stimulus. In particular, 25 target genes are involved in regulating plant hormone signal transduction and metabolism, indicating that these associationmicroRNAs may play important regulatory roles in responding to PEG resistance. Moreover, this study adds a significant number of novel miRNAs to the tobacco miRNome.

分类号： Q78

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